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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 383 - 391 (9page)
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초록· 키워드
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It is controversial whether type I collagen itself can maintain and improve chondrogenic phenotype of chondrocytes in a three-dimensional (3D) environment. In this study, we examined the effect of type I collagen concentration in hydrogel (0.5, 1, and 2 mg/ml) on the growth and phenotype expression of rat chondrocytes in vitro. All collagen hydrogels showed substantial contractions during culture, in a concentration-dependent manner, which was due to the cell proliferation. The cell viability was shown to be the highest in 2 mg/ml collagen gel. The mRNA expression of chondrogenic phenotypes, including SOX9, type II collagen, and aggrecan, was significantly up-regulated, particularly in 1 mg/ml collagen gel. Furthermore, the production of type II collagen and glycosaminoglycan (GAG) content was also enhanced. The results suggest that type I collagen hydrogel is not detrimental to, but may be useful for, the chondrocyte culture for cartilage tissue engineering.
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참고문헌 (40)
참고문헌 신청
Huang AH / 2010 / Mechanics and mechanobiology of mesenchymal stem cell-based engineered cartilage / J Biomech 43:128~136
Pulkkinen HJ / 2010 / Engineering of cartilage in recombinant human type II collagen gel in nude mouse model in vivo / Osteoarthritis Cartilage 18:1077~1087
Pulkkinen HJ / 2008 / Recombinant human type II collagen as a material for cartilage tissue engineering / Int J Artif Organs 31:960~969
Ricard-Blum S / 2011 / The collagen family / Cold Spring Harb Perspect Biol 3:a004978
Ignatius A / 2005 / Tissue engineering of bone : effects of mechanical strain on osteoblastic cells in type I collagen matrices / Biomaterials 26:311~318
Pulkkinen HJ / 2010 / Engineering of cartilage in recombinant human type II collagen gel in nude mouse model in vivo / Osteoarthritis Cartilage 18:1077~1087
Pulkkinen HJ / 2008 / Recombinant human type II collagen as a material for cartilage tissue engineering / Int J Artif Organs 31:960~969
Ricard-Blum S / 2011 / The collagen family / Cold Spring Harb Perspect Biol 3:a004978
Ignatius A / 2005 / Tissue engineering of bone : effects of mechanical strain on osteoblastic cells in type I collagen matrices / Biomaterials 26:311~318
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