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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 527 - 537 (11page)
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Three-dimensional (3D) cultures are known to promote cell differentiation. Previously, we investigated the differentiation of rat dermal fibroblasts to α-smooth muscle actin (α-SMA)-positive myofibroblasts through transforming growth factor (TGF)-β production using a 3D culture model. Here, we investigated the phenotypic change from dermal mesenchymal cells (mostly fibroblasts) to osteoblast-like cells, being inspired by the roles of smooth muscle cells or fibroblasts during vascular calcification. Spindle-shaped cells that grew in heterologous populations out of dermal explants from 2-day-old Wistar rats were cultured within a collagen matrix. α-SMA and alkaline phosphatase (ALP) messenger RNA (mRNA) levels initially increased, followed by a rise in Runx2 and osteocalcin (OCN) mRNA levels without calcification. Calcium deposits were produced in the presence of a high concentration of inorganic phosphate (2.1 mM) or β-glycerophosphate (βGP, 10 mM) after 2 weeks of culture, and both were sensitive to an inhibitor of type III phosphate transporters. An ALP inhibitor decreased only βGP-induced calcification. Inhibition of TGF-β type-I receptors attenuated ALP mRNA levels and βGP-induced calcification, suggesting that endogenous TGF-β stimulates ALP activity and then βGP breakdown. An increase in the number of cells embedded in the collagen gel enhanced the mRNA levels of Runx2 and OCN, but not of ALP. Collectively, several factors are likely to promote the differentiation of dermal mesenchymal cells into osteoblast-like cells and ectopic calcification in a 3D collagen matrix, implying the utility of these cells as a potential autologous cell source for tissue engineering.
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참고문헌 (30)
참고문헌 신청
Buttery L / 2011 / Osteogenic differentiation of embryonic stem cells in 2D and 3D culture / Methods Mol Biol 695:281~308
Matthews BG / 2014 / Enhanced osteoblastogenesis in three-dimensional collagen gels / Bonekey Rep 3:560
Hata S / 2014 / Proteolytic and non-proteolytic activation of keratinocyte-derived latent TGF-β1 induces fibroblast differentiation in a wound-healing model using rat skin / J Pharmacol Sci 124:230~243
Gabbiani G / 1971 / Presence of modified fibroblasts in granulation tissue and their possible role in wound contraction / Experientia 27:549~550
Tomasek JJ / 2002 / Myofibroblasts and mechano-regulation of connective tissue remodelling / Nat Rev Mol Cell Biol 3:349~363
Matthews BG / 2014 / Enhanced osteoblastogenesis in three-dimensional collagen gels / Bonekey Rep 3:560
Hata S / 2014 / Proteolytic and non-proteolytic activation of keratinocyte-derived latent TGF-β1 induces fibroblast differentiation in a wound-healing model using rat skin / J Pharmacol Sci 124:230~243
Gabbiani G / 1971 / Presence of modified fibroblasts in granulation tissue and their possible role in wound contraction / Experientia 27:549~550
Tomasek JJ / 2002 / Myofibroblasts and mechano-regulation of connective tissue remodelling / Nat Rev Mol Cell Biol 3:349~363
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