지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 445 - 452 (8page)
이용수
초록· 키워드
오류제보하기
BACKGROUND: Cell-based therapies for treating bone defects require a source of stem cells with osteogenic potential. There is evidence from pathologic ossification within muscles that human skeletal muscles contain osteogenic progenitor cells. However, muscle samples are usually acquired through a traumatic biopsy procedure which causes pain and morbidity to the donor. Herein, we identified a new alternative source of skeletal muscle stem cells (SMSCs) without conferring morbidity to donors. METHODS: Adherent cells isolated from human orbicularis oculi muscle (OOM) fragments, which are currently discarded during ophthalmic cosmetic surgeries, were obtained using a two-step plating method. The cell growth kinetics, immunophenotype and capabilities of in vitro multilineage differentiation were evaluated respectively. Moreover, the osteogenically-induced cells were transduced with GFP gene, loaded onto the porous b-tricalcium phosphate (b-TCP) bioceramics, and transplanted into the subcutaneous site of athymic mice. Ectopic bone formation was assessed and the cell fate in vivo was detected. RESULTS: OOM-derived cells were fibroblastic in shape, clonogenic in growth, and displayed phenotypic and behavioral characteristics similar to SMSCs. In particular, these cells could be induced into osteoblasts in vitro evidenced by the extracellular matrix calcification and enhanced alkaline phosphatase (ALP) activity and osteocalcin (OCN) production. New bone formation was found in the cell-loaded bioceramics 6 weeks after implantation. By using the GFP-labeling technique, these muscle cells were detected to participate in the process of ectopic osteogenesis in vivo. CONCLUSION: Our data suggest that human OOM tissue is a valuable and noninvasive resource for osteoprogenitor cells to be used in bone repair and regeneration.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Liu G / 2007 / Tissue-engineered bone formation using human bone marrow stromal cells and novel ${\beta}$ -tricalcium phosphate / Biomed Mater 2:78~86
Mastrogiacomo M / 2005 / Bone and cartilage formation by skeletal muscle derived cells / J Cell Physiol 204:594~603
Sun JS / 2005 / The role of muscle-derived stem cells in bone tissue engineering / Biomaterials 26:3953~3960
Levy MM / 2001 / Osteoprogenitor cells of mature human skeletal muscle tissue: an in vitro study / Bone 29:317~322
Gao X / 2014 / A comparison of bone regeneration with human mesenchymal stem cells and muscle-derived stem cells and the critical role of BMP / Biomaterials 35:6859~6870
Mastrogiacomo M / 2005 / Bone and cartilage formation by skeletal muscle derived cells / J Cell Physiol 204:594~603
Sun JS / 2005 / The role of muscle-derived stem cells in bone tissue engineering / Biomaterials 26:3953~3960
Levy MM / 2001 / Osteoprogenitor cells of mature human skeletal muscle tissue: an in vitro study / Bone 29:317~322
Gao X / 2014 / A comparison of bone regeneration with human mesenchymal stem cells and muscle-derived stem cells and the critical role of BMP / Biomaterials 35:6859~6870
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of culture strategies for stem cell maintenance and differentiation through regulation of cell behaviors
한국생물공학회 학술대회
2016 .04
-
Development of Novel Suspending Cell Culture Substrates for ex vivo Cell-gene Therapy
한국생물공학회 학술대회
2018 .04
-
The Osteogenic Role of Biomaterials Combined with Human-Derived Dental Stem Cells in Bone Tissue Regeneration
조직공학과 재생의학
2023 .04
-
심근유래의 줄기세포 프라이밍 및 조직공학 기술에 의한 심혈관 재생 전략
KSBB Journal
2018 .09
-
FGF2-Mediated Insulin-Producing Beta Cells Differentiated from Human Omentum-Derived Stem Cells
한국생물공학회 학술대회
2022 .04
-
Guiding osteogenesis of mesenchymal stem cells using carbon-based nanomaterials
Nano Convergence
2017 .01
-
Osteogenic Induction Capability of Runx2-Conjugated 30kc19α in Mesenchymal Stem Cells
한국생물공학회 학술대회
2019 .10
-
Development of Next-generation Cell-based Therapeutics for Diabetes
한국생물공학회 학술대회
2023 .04
-
Human Cell Atlas and cell-type authentication for regenerative medicine
Experimental and Molecular Medicine
2020 .09
-
Three-Dimensional Cell Culture Using Modified Natural Polymer for Cell Therapy
한국생물공학회 학술대회
2020 .06
-
Osteogenic Differentiation of Human Mesenchymal Stem Cells Using Osteoblast Extracellular Matrix Incorporated into PEG Scaffold
한국생물공학회 학술대회
2018 .04
-
골격근 분화과정에서의 세포 견인력 분석 기반 세포 불균일성 가시화
한국가시화정보학회 학술발표대회 논문집
2019 .06
-
Endogenous Stem Cells in Homeostasis and Aging
조직공학과 재생의학
2017 .01
-
Direct conversion of fibroblasts to osteoblasts as a novel strategy for bone regeneration in elderly individuals
Experimental and Molecular Medicine
2019 .01
-
Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells by Regulating the Tension between the Cell and Surface Using Magnetic Nanoparticle-incorporated Cells
한국생물공학회 학술대회
2019 .10
-
Development of in vitro Human Skeletal Muscle Models Using Microfabricated Devices
한국생물공학회 학술대회
2021 .04
-
The promotive effect of human induced pluripotent stem cell-conditioned medium on growth and migration of human skin cells
한국생물공학회 학술대회
2016 .10
-
Effects of Uniaxial Cyclic Stretch Loading on Morphology of Adipose Derived Stem Cells
조직공학과 재생의학
2016 .01
-
Development of 3D-shaped Cell Delivery Method for Tissue Regeneration
한국생물공학회 학술대회
2023 .10
-
Coculture Effects on the Osteogenic Differentiation of Human Mesenchymal Stromal Cells
조직공학과 재생의학
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0