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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 231 - 238 (8page)
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Type I collagen is the most abundant extracellular matrix in bone and dentin. It was reported to be effective in inducing the osteoblast differentiation. Majority of the type I collagen used in scientific research are from bovine skin, porcine skin and rat tail. Recently, a novel type I collagen possessing sound thermal stability, was isolated from tilapia fish scale. It was reported to be able to induce the differentiation of human mesenchymal stem cells into osteoblasts. However, no information is available regarding its effect on odontoblast-like cells. The purpose of this study was to investigate the effect of type I collagen derived from tilapia fish scale on odontoblast-like cells. Type I collagen was immobilized to tissue culture polystyrene dish. Biocompatibility of type I collagen was evaluated in terms of initial cell number, alkaline phosphatase activity (ALP activity), odontogenic gene expression and calcific deposition. The results showed that type I collagen purified from tilapia fish scale significantly enhanced the cell attachment to nearly twofold as compared to control; moreover, cells cultured in type I collagen-modified substrate was induced to differentiate toward odontoblast lineage as demonstrated by upregulation of ALP activity on day 7, enhancement of ALP, BSP mRNA expression on day 7 and 10, and accelerated mineralization on day 9. It is therefore suggested that type I collagen derived from tilapia scale, an underutilized resource, holds promise as scaffolding material in the application of tissue engineering in dental field.
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참고문헌 (25)
참고문헌 신청
Kadler KE / 2007 / Collagens at a glance / J Cell Sci 120(Pt 12):1955~1958
Mizuno M / 2003 / Type I collagen regulated dentin matrix protein-1(Dmp-1)and osteocalcin(OCN)gene expression of rat dental pulp cells / J Cell Biochem 88:1112~1119
Saito T / 2000 / In vitro apatite induction by phosphophoryn immobilized on modified collagen fibrils / J Bone Miner Res 15:1615~1619
Primorac D / 2014 / Osteogenesis imperfecta--multi-systemic and life-long disease that affects whole family / Coll Antropol 38:767~772
Cabral WA / 2014 / Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta / PLoS Genet 10:e1004465
Mizuno M / 2003 / Type I collagen regulated dentin matrix protein-1(Dmp-1)and osteocalcin(OCN)gene expression of rat dental pulp cells / J Cell Biochem 88:1112~1119
Saito T / 2000 / In vitro apatite induction by phosphophoryn immobilized on modified collagen fibrils / J Bone Miner Res 15:1615~1619
Primorac D / 2014 / Osteogenesis imperfecta--multi-systemic and life-long disease that affects whole family / Coll Antropol 38:767~772
Cabral WA / 2014 / Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta / PLoS Genet 10:e1004465
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