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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 143 - 154 (12page)
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초록· 키워드
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The bloody clam, Tegillarca granosa, is a commercial benthic bivalve, having a strong accumulation ability and torrelence to cadmium. To investigate whether vitellogenin (Vg) is involved in cadmium (Cd) detoxification, the full-length cDNA of T. granosa Vg was cloned, and its expression pattern in response to cadmium exposure was studied compared with the reference metallothionein (MT) gene. The full T. granosa Vg sequence consisted of 8988 bp, including a 6930-bp open reading frame that encoded a 2309 amino acid polypeptide. The deduced Vg protein contained a Vg N-terminal domain, domain of unknown function (DUF1943), SbcC domain, and von Willebrand factor typeD domain (VWD). Multiple metal-binding sites were predicted in the deduced T. granosaVg protein, suggesting its potential in functioning as a metal-binding protein. In addition, Vg expression increased in the T. granosa digestive gland and hemolymph in time-dependent manner after exposure to 1, 3, 6 and 9 lg/L Cd for 28 days.MTexpression was measured in parallel with Vg expression, and the latter was more sensitive to Cd induction than the former. Together, results of the present research suggested that Vg may play an important role in T. granosa metal detoxification.
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참고문헌 (63)
참고문헌 신청
Aly W / 2014 / Limitations of metallothioneins in common cockles (Cerastoderma edule) and sponges (Haliclona oculata) as biomarkers of metal contamination in a semi-enclosed coastal area / Sci Total Environ 473?474:391~397
Auclair J / 2014 / Biochemical ecotoxicology (principles and methods) / Elsevier :75~101
Babin PJ / 1999 / Apolipophorin II/I, apolipoprotein B, vitellogenin and microsomal triglyceride transfer protein genes are derived from a common ancestor / J Mol Evol 49:150~160
Baker ME / 1988 / Is vitellogenin an ancestor of apolipoprotein-B-100of human low-density lipoprotein and human lipoprotein-lipase / Biochemistry 255:1057~1060
Banni M / 2005 / Monitoring pollution in Tunisian coasts:application of a classification scale based on biochemical markers / Biomarkers 10:105~116
Auclair J / 2014 / Biochemical ecotoxicology (principles and methods) / Elsevier :75~101
Babin PJ / 1999 / Apolipophorin II/I, apolipoprotein B, vitellogenin and microsomal triglyceride transfer protein genes are derived from a common ancestor / J Mol Evol 49:150~160
Baker ME / 1988 / Is vitellogenin an ancestor of apolipoprotein-B-100of human low-density lipoprotein and human lipoprotein-lipase / Biochemistry 255:1057~1060
Banni M / 2005 / Monitoring pollution in Tunisian coasts:application of a classification scale based on biochemical markers / Biomarkers 10:105~116
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