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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 767 - 777 (11page)
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Few studies have attempted to characterize the pheasant (Phasianus colchicus) immunoglobulin Y (IgY) heavy chain constant region. In the present study, fragments of the pheasant IgY heavy chain constant region were cloned, analyzed, and expressed. The cross-reactivity of IgY or immunoglobulin G (IgG)s with antigens from other vertebrate species was determined using dot-enzymelinked immunosorbent assay and western blot analysis. Five peptides of the pheasant IgY heavy chain constant region were synthesized to determine its immunoregulatory activity in vitro. The IgY heavy chain constant region from pheasant showed the highest homology with that from chicken (71.2 %) and duck (49.1 %). Phylogenetic analysis for IgY showed that pheasant was closely related to chicken and duck than to any other analyzed vertebrate species. The rabbit anti-chicken IgG showed immunologic cross-reactivity with recombinant proteins of the pheasant IgY heavy chain constant region. Four peptides were able to induce significant up-regulation of interleukin (IL)-1b, IL-4, and interferon-c in chicken peripheral blood lymphocytes, suggesting a new role of avian IgY in immune regulation.
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참고문헌 (36)
참고문헌 신청
Annamalai T / 2012 / Interleukin 4 increases CCR9 expression and homing of lymphocytes to gut-associated lymphoid tissue in chickens / Vet Immunol Immunopathol 145:257~263
Choi JW / 2010 / Molecular cloning and comparative analysis of immunoglobulin heavy chain genes from Phasianus colchicus, Meleagris gallopavo, and Coturnix japonica / Vet Immunol Immunopathol 136:248~256
Cray C / 2008 / Cross-reactivity of anti-chicken IgY antibody with immunoglobulins of exotic avian species / Vet Clin Pathol 37:328~331
Davison F / 2008 / Struction and evolution of avian immunoglobulins / Elsevier Ltd :107~128
Digby MR / 1995 / Cloning and expression of the chicken interferon-gamma gene / J Interferon Cytokine Res 15:939~945
Choi JW / 2010 / Molecular cloning and comparative analysis of immunoglobulin heavy chain genes from Phasianus colchicus, Meleagris gallopavo, and Coturnix japonica / Vet Immunol Immunopathol 136:248~256
Cray C / 2008 / Cross-reactivity of anti-chicken IgY antibody with immunoglobulins of exotic avian species / Vet Clin Pathol 37:328~331
Davison F / 2008 / Struction and evolution of avian immunoglobulins / Elsevier Ltd :107~128
Digby MR / 1995 / Cloning and expression of the chicken interferon-gamma gene / J Interferon Cytokine Res 15:939~945
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