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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 100 - 108 (9page)
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초록· 키워드
오류제보하기
A detailed knowledge about the mode of enzyme action is critical to get better understanding the productivity of target molecules. The present study was then performed to elucidate the mode of enzyme action and hydrolysis pattern of chitosanase toward high molecular weight chitosan. Hydrolysates derived from the reaction condition were then analyzed by Matrix Associated Laser Desorption Ionization - Time of Flight mass spectrometry (MALDI-TOF MS) analysis to define the real size of hydrolysates. Also, the sequence of linking pattern of hydrolysates derived from the action of chitosanase was determined based on the identification of both glucosamine (GlcN) and N-acetylglucosamine (GlcNAc). The results demonstrate that both homogeneous and heterogeneous chitooligosaccharides, designated as active molecular chitosan (AMC) can be produced without such a big differences. According to these results, we suggest that GlcNAc may not be a terminator for enzymatic hydrolysis processing as a key molecule in chitosanase reaction to determine the length of AMC. Upon identifying the type of linkage and size of AMC, nevertheless, these can be used as potent materials in various research fields including cosmeceutical and pharmaceutical applications.
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등록된 정보가 없습니다.
참고문헌 (28)
참고문헌 신청
Nanjo, F / 1991 / Enzymatic method for determination of the degree of deacetylation of chitosan / Anal. Biochem 193:164~167
Azuma, K. / 2015 / Anticancer and anti-inflammatory properties of chitin and chitosan oligosaccharides / J. Funct. Biomater 6:33~49
Gibot, L / 2015 / Anticancer properties of chitosan on human melanoma are cell line dependent / Int. J. Biol. Macromol 72:370~379
Karagozlu, M. Z / 2014 / Anticancer effects of chitin and chitosan derivatives / Adv. Food Nutr. Res 72:215~225
Badawy, M. E / 2016 / The antibacterial activity of chitosan products blended with monoterpenes and their biofilms against plant pathogenic bacteria / Scientifica (Cairo) 2016:1796256
Azuma, K. / 2015 / Anticancer and anti-inflammatory properties of chitin and chitosan oligosaccharides / J. Funct. Biomater 6:33~49
Gibot, L / 2015 / Anticancer properties of chitosan on human melanoma are cell line dependent / Int. J. Biol. Macromol 72:370~379
Karagozlu, M. Z / 2014 / Anticancer effects of chitin and chitosan derivatives / Adv. Food Nutr. Res 72:215~225
Badawy, M. E / 2016 / The antibacterial activity of chitosan products blended with monoterpenes and their biofilms against plant pathogenic bacteria / Scientifica (Cairo) 2016:1796256
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