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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 118 - 126 (9page)
- DOI
- 10.17642/jcc.26.3.4
이용수
초록· 키워드
오류제보하기
The complex of chitosan and hyaluronic acid was treated with enzyme to obtain chitosan oligosaccharide designated to COS hereafter. The degree of decomposition was confirmed by reducing sugar quantitative method, and the molecular weight size was confirmed by Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) mass spectrometry analysis. Based on the fact that the substrate specificity of the enzyme is highly dependent on temperature, substrate concentration, and pH, etc, the COS obtained by treating a mixture of chitosan and hyaluronic acid, which physically mixes well, under the non-optimal reaction conditions of the chitosanase. Accordingly, it exhibited very significant antibacterial activity against Meticillin Resistant Stapylococcs aureus (MRSA). As a method for controlling molecular weight of COS, it was intended to propose a new enzyme usage as a study to identify the characteristics of the hydrolysates according to the reaction delay and enzyme activity through complex formation.
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등록된 정보가 없습니다.
참고문헌 (38)
참고문헌 신청
Knidri, H. E. / 2019 / Rapid and efficient extraction of chitin and chitosan for scale-up production: Effect of process parameters on deacetylation degree and molecular weight / Int. J. Biol. Macromol 139:1092~1102
Yuan, X. / 2019 / A review on the preparation of chitosan oligosaccharides and application to human health, animal husbandry and agricultural production / Carbohydr. Polym 220:60~70
Qin, Z. / 2018 / Expression and characterization of a novel cold-adapted chitosanase suitable for chitooligosaccharides controllable preparation / Food Chem 253:139~147
Thadathil, N. / 2014 / Recent developments in chitosanase research and its biotechnological applications: a review / Food Chem 150:392~399
조인후 / 2015 / Optimization of High Hydrostatic Pressure Enzymatic Hydrolysis from Flatfish Byproducts using Response Surface Methodology / Journal of Chitin and Chitosan 20(2):106~114
Yuan, X. / 2019 / A review on the preparation of chitosan oligosaccharides and application to human health, animal husbandry and agricultural production / Carbohydr. Polym 220:60~70
Qin, Z. / 2018 / Expression and characterization of a novel cold-adapted chitosanase suitable for chitooligosaccharides controllable preparation / Food Chem 253:139~147
Thadathil, N. / 2014 / Recent developments in chitosanase research and its biotechnological applications: a review / Food Chem 150:392~399
조인후 / 2015 / Optimization of High Hydrostatic Pressure Enzymatic Hydrolysis from Flatfish Byproducts using Response Surface Methodology / Journal of Chitin and Chitosan 20(2):106~114
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