지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 1,392 - 1,396 (5page)
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초록· 키워드
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Neuroblastoma (NB) is the most common extra-cranial solid tumor of childhood and is characterized by a wide range of clinical behaviors. Amplification of MYCN is a well-known poor prognostic factor in NB patients. As the MYCN amplification status is usually tested using tumor specimens, lengthy and invasive procedures are unavoidable. To evaluate the possibility of detecting MYCN amplification without invasive procedure, we performed conventional polymerase chain reaction (PCR) analysis to identify MYCN amplification using the preserved serum DNA. PCR of serum DNA was done in 105 NB patients whose MYCN status had been confirmed by fluorescence in situ hybridization. MYCN amplification was evaluated as the ratio of signal intensities between MYCN and NAGK (M/N ratio). When regarding the tissue FISH results as a reference, 10 patients had MYCNamplified (MNA) NB, and 95 had non-MNA NB. The M/N ratio of the MNA group (median 2.56, range 1.01-3.58) was significantly higher than that of the non-MNA group (median 0.97, range 0.67-5.18) (P < 0.001). In the receiver operating characteristic curve analysis, the area under the curve was 0.957 (95% confidence interval 0.898-1.000; P < 0.001), and it showed 90.9% sensitivity and 97.9% specificity with the selected cut-off value set as 1.6. The detection of MYCN amplification using conventional PCR analysis of serum samples seems to be a simple and promising method to evaluate the MYCN status of NB patients. Further study with a larger set of patients is needed to confirm the accuracy of this result.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Cohn SL / 2009 / The International Neuroblastoma Risk Group(INRG)classification system : an INRG Task Force report / J Clin Oncol 27:289~297
Owens C / 2012 / Neuroblastoma : the impact of biology and cooperation leading to personalized treatments / Crit Rev Clin Lab Sci 49:85~115
Park JR / 2013 / Children’s Oncology Group’s 2013 blueprint for research: neuroblastoma / Pediatr Blood Cancer 60:985~993
Brodeur GM / 1984 / Amplification of N-myc in untreated human neuroblastomas correlates with advanced disease stage / Science 224:1121~1124
Janoueix-Lerosey I / 2009 / Overall genomic pattern is a predictor of outcome in neuroblastoma / J Clin Oncol 27:1026~1033
Owens C / 2012 / Neuroblastoma : the impact of biology and cooperation leading to personalized treatments / Crit Rev Clin Lab Sci 49:85~115
Park JR / 2013 / Children’s Oncology Group’s 2013 blueprint for research: neuroblastoma / Pediatr Blood Cancer 60:985~993
Brodeur GM / 1984 / Amplification of N-myc in untreated human neuroblastomas correlates with advanced disease stage / Science 224:1121~1124
Janoueix-Lerosey I / 2009 / Overall genomic pattern is a predictor of outcome in neuroblastoma / J Clin Oncol 27:1026~1033
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