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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 269 - 281 (13page)
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With the continual development of genetically modified (GM) crops, it has become necessary to develop detailed and effective molecular characterization methods to select candidate events from a large pool of transformation events. Relative to traditional molecular analysis methods such as the polymerase chain reaction (PCR) and Southern blot hybridization, next generation sequencing (NGS) technology for whole-genome sequencing of complex crop genomes had proven comparatively useful for in-depth molecular characterization. In this study, four transformation events, including one in Bacillus thuringiensis (Bt)-resistant rice, one in resveratrol-producing rice, and two in beta-carotene-enhanced soybeans, were selected for molecular characterization. To merge NGS analysis and Southern blot-hybridization results, we confirmed the transgene insertion sites, insertion construction, and insertion numbers of these four transformation events. In addition, the read-coverage depth assessed by NGS analysis for inserted genes might provide consistent results in terms of inserted T-DNA numbers in case of complex insertion structures and highly duplicated donor genomes; however, PCR-based methods can produce incorrect conclusions. Our combined method provides an effective and complete analytical approach for whole-genome visual inspection of transformation events that require biosafety assessment.
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등록된 정보가 없습니다.
참고문헌 (3)
참고문헌 신청
박두리 / 2015 / Efficiency to Discovery Transgenic Loci in GM Rice Using Next Generation Sequencing Whole Genome Re-sequencing / Genomics & Informatics 13(3):81~85
Yang Qin / 2013 / Molecular Characterization of Transgenic Rice Producing Resveratrol / Plant Breeding and Biotechnology 1(4):406~415
친양 / 2015 / 형질전환 베타카로틴 강화 콩 계통 선발 및 도입유전자 특성 분석 / 한국육종학회지 47(2):111~117
Yang Qin / 2013 / Molecular Characterization of Transgenic Rice Producing Resveratrol / Plant Breeding and Biotechnology 1(4):406~415
친양 / 2015 / 형질전환 베타카로틴 강화 콩 계통 선발 및 도입유전자 특성 분석 / 한국육종학회지 47(2):111~117
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