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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The β-carotene biofortified transgenic rice was developed by transforming rice cv. Nakdongbyeo with phytoenesynthase (Psy) and carotene desaturase (Crt I) genes isolated from Capsicum and Pantoea. The aim of this study was to performmolecular characterization of rice transformants of T5-T7 generation harboring Psy and Ctr I genes driven by endosperm specificglobulin promoter for biosafety evaluation of β-carotene biofortified transgenic rice. The structure and sequence of T-DNA inthe transformation vector and the insertion sites, flanking sequences and generational stability of inserted T-DNA in transgenicrice lines were analyzed. The transformation vector consisted of right border, MAR gene, carotenogenic genes unit, herbicideresistance selectable marker unit, MAR gene and left border in sequential order. T-DNA was introduced at the position of30,363,938-30,363,973 bp of chromosome No. 2 by adaptor-ligation PCR. Stable integration of T-DNA and stable expression ofbar gene was confirmed in T5 to T7 generations. It was also confirmed that the backbone DNA of transformation vectorcontaining antibacterial gene was not present in the genome of β-carotene biofortified transgenic rice. HPLC analysis confirmedthat carotenoids were consistently detected through T5-T7 generations.
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참고문헌 (15)
참고문헌 신청
/ / Korea Biosafety Clearing House
Brookes G / 2007 / Global impact of biotech crops: Socio-economic and environmental effects in the first ten years of commercial use / AgBioForum 9 : 139 ~ 151
De Vries J / 2004 / Microbial horizontal gene transfer and the DNA release from transgenic crop plants / Plant and Soil 266 : 91 ~ 104
Ha SH / 2010 / Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm / Plant Biotechnol. J 8 : 928 ~ 938
Hoa T / 2003 / Golden Indica and Japonica rice lines amenable to deregulation / Plant Physiol 133 : 161 ~ 169
Brookes G / 2007 / Global impact of biotech crops: Socio-economic and environmental effects in the first ten years of commercial use / AgBioForum 9 : 139 ~ 151
De Vries J / 2004 / Microbial horizontal gene transfer and the DNA release from transgenic crop plants / Plant and Soil 266 : 91 ~ 104
Ha SH / 2010 / Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm / Plant Biotechnol. J 8 : 928 ~ 938
Hoa T / 2003 / Golden Indica and Japonica rice lines amenable to deregulation / Plant Physiol 133 : 161 ~ 169
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