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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 341 - 346 (6page)
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초록· 키워드
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The objectives of this study were to 1) evaluate the efficiency of the protocol of Agrobacterium-mediated transformation of cucumber to introduce phytoene synthase-2a carotene desaturase (PAC genes); 2) demonstrate the integration of PAC genes into the genome of putative transgenic cucumber based on growth on selection medium, PCR and Southern analysis; 3) evaluate the expression of PAC genes in transgenic cucumber based on the analysis of RT-PCR and Northern blot hybridization. Out of 5,945 cotyledonary-node explants inoculated with Agrobacterium, 65 (1.1%) explants produced 238 shoots. Integration of PAC genes into the genome of the cucumber was demonstrated based on the analysis of gDNA- PCR, 21 out of the 238 plants regenerated; while 6 plants proved positive for Southern blot hybridization. Transgene expression was demonstrated based on analysis of RT-PCR, 6 plants proved positive out of the 6 plants analyzed; while 4 plants out of 6 proved positive during Northern blot hy bridization. This study successfully demonstrated the production of transgenic cucumber, integration, and expression of the PAC gene in cucumber.
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등록된 정보가 없습니다.
참고문헌 (38)
참고문헌 신청
Chee PP / 1990 / Transformation of Cucumis sativus tissue by Agrobacterium tumefaciens and the regeneration of transformed plants / Plant Cell Rep 9:245~248
조미애 / 2005 / 오이의 형질전환을 위한 선발마커로서 Glufosinate의 이용 / Journal of Plant Biotechnology 32(3):161~165
조미애 / 2005 / Agrobacterium 공동 배양을 통한 자엽절 절편 배양으로부터 멜론 형질전환체 생산 / Journal of Plant Biotechnology 32(4):257~261
Dellaporta SL / 1983 / Molecular Biology of Plants: A laboratory Course Manual / Cold Spring Harbor Laboratory, Cold Spring Harbor :36~37
Dong JZ / 1991 / Transformation of melon (Cucumis melo L.) and expression from the cauliflower mosaic virus 35S promoter in transgenic melon plants / Biotechnology 9:858~863
조미애 / 2005 / 오이의 형질전환을 위한 선발마커로서 Glufosinate의 이용 / Journal of Plant Biotechnology 32(3):161~165
조미애 / 2005 / Agrobacterium 공동 배양을 통한 자엽절 절편 배양으로부터 멜론 형질전환체 생산 / Journal of Plant Biotechnology 32(4):257~261
Dellaporta SL / 1983 / Molecular Biology of Plants: A laboratory Course Manual / Cold Spring Harbor Laboratory, Cold Spring Harbor :36~37
Dong JZ / 1991 / Transformation of melon (Cucumis melo L.) and expression from the cauliflower mosaic virus 35S promoter in transgenic melon plants / Biotechnology 9:858~863
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