지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 406 - 415 (10page)
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초록· 키워드
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Resveratrol, a plant phenolic compound, has potential therapeutic benefits due to its antioxidant properties. This is substantiated by previous studies that show that resveratrol derived from rice grains is an effective treatment agent for metabolic syndrome. Here, we characterized the T-DNA sequence, inserted T-DNA structure, copy number, integrity of the transgene locus,resveratrol synthase gene expression and resveratrol contents in the grains of two resveratrol transgenic rice lines, Iksan515 and Iksan526. The T-DNA transformation vector contained two expression cassettes of the resveratrol synthase gene under the control of the ubiquitin promoter and the bar selection marker gene under the control of the CaMV35S promoter. Flanking sequence analysis indicated that the T-DNAs were inserted into intergenic regions of chromosome 4 for Iksan515 and chromosome 12 for Iksan526. Two T-DNAs connected in an inverted repeat structure at a single locus of the rice genome were identified by whole genome sequencing and Southern blot hybridization in both Iksan515 and Iksan526. No novel open reading frames (ORFs) around insertion sites, sequences encoding allergenic or toxic protein, or other unintended effects by T-DNA insertion were found in either case. In addition, resveratrol synthase gene expression in leaves and resveratrol detection in brown rice grains suggested the successful expression of the inserted foreign resveratrol synthase gene in two transgenic rice lines.
Keywords Molecular characterization, Resveratrol, T-DNA,
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참고문헌 (29)
참고문헌 신청
Athar M / 2007 / Resveratrol: a review of preclinical studies for human cancer protection / Toxicol. Appl. Pharmacol 224:274~283
Baek SH / 2013 / Creation of Resveratrol-Enriched Rice for the Treatment of Metabolic Syndrome and Related Diseases / PLoS One 8:e57930
Balzergue S / 2001 / Improved PCR-walking for large-scale isolation of plant T-DNA borders / Biotechniques 30:496~504
Baur JA / 2006 / Therapeutic potential of resveratrol: the in vivo evidence / Nat. Rev. Drug Discov 5:493~506
Chen DH / 1999 / A rapid DNA minipreparation method suitable for AFLP and other PCR applications / Plant Mol. Biol. Rep 17:53~57
Baek SH / 2013 / Creation of Resveratrol-Enriched Rice for the Treatment of Metabolic Syndrome and Related Diseases / PLoS One 8:e57930
Balzergue S / 2001 / Improved PCR-walking for large-scale isolation of plant T-DNA borders / Biotechniques 30:496~504
Baur JA / 2006 / Therapeutic potential of resveratrol: the in vivo evidence / Nat. Rev. Drug Discov 5:493~506
Chen DH / 1999 / A rapid DNA minipreparation method suitable for AFLP and other PCR applications / Plant Mol. Biol. Rep 17:53~57
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