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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 309 - 315 (7page)
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초록· 키워드
오류제보하기
Advances in biotechnology have led to progress in crop genetic engineering to improve agricultural productivity. The use of genetically modified (GM) crops has increased, as have consumers’ and regulators’ concerns about the safety of GM crops to human health, and ecological biodiversity. As such, the identification of GM crops is a critical issue for developers and distributors, and their labeling is mandatory. Multiplex polymerase chain reaction (PCR) has been developed and its use validated for the detection and identification of GM crops in quarantine. Herein, we established a simultaneous detection method to identify four GM maize events. Eventspecific primers were designed between the junction region of transgene and genome of four GM maize lines, namely 5307, DAS-40278-9, MON87460, and MON87427. To verify the efficiency and accuracy of the multiplex PCR we used specificity analysis, limit of detection evaluation, and mixed certified reference materials identification. The multiplex PCR method was applied to analyze 29 living, modified maize volunteers collected in South Korea in 2018 and 2019. We performed multiplex PCR analysis to identify events and confirmed the result by simplex PCR using each eventspecific primer. As a result, rather than detecting each event individually, the simultaneous detection PCR method enabled the rapid analysis of 29 GM maize volunteers. Thus, the novel multiplex PCR method is applicable for living modified organism volunteer identification.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Basak S / 2014 / Detection and identification of transgenic elements by fluorescent-PCR-based capillary gel electrophoresis in genetically modified cotton and soybean / J. AOAC Int 97(1):159~165
Bhoge RK / 2015 / Event-specific analytical methods for six genetically modified maize events using visual and real-time loopmediated isothermal amplification / Food Control 55:18~30
Chen L / 2011 / Development of the visual loop-mediated isothermal amplification assays for seven genetically modified maize events and their application in practical samples analysis / J. Agric. Food Chem 59(11):5914~5918
Cottenet G / 2013 / Development and validation of a multiplex real-time PCR method to simultaneously detect 47 targets for the identification of genetically modified organisms / Anal. Bioanal. Chem 405(21):6831~6844
Dobnik D / 2018 / Methods Mol. Biol. 1768 / Humana Press :69~98
Bhoge RK / 2015 / Event-specific analytical methods for six genetically modified maize events using visual and real-time loopmediated isothermal amplification / Food Control 55:18~30
Chen L / 2011 / Development of the visual loop-mediated isothermal amplification assays for seven genetically modified maize events and their application in practical samples analysis / J. Agric. Food Chem 59(11):5914~5918
Cottenet G / 2013 / Development and validation of a multiplex real-time PCR method to simultaneously detect 47 targets for the identification of genetically modified organisms / Anal. Bioanal. Chem 405(21):6831~6844
Dobnik D / 2018 / Methods Mol. Biol. 1768 / Humana Press :69~98
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