지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 질병관리본부 Osong Public Health and Research Persptectives Osong Public Health and Research Persptectives Vol.11 No.1
- 발행연도
- 2020.1
- 수록면
- 53 - 59 (7page)
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초록· 키워드
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Objectives Carbapenem resistance is a serious clinical and public health threat. Carbapenemase can confer carbapenem resistance, and most carbapenemase genes are plasmid encoded so resistance can easily spread. In this study, we aimed to develop a novel system based on the TaqMan platform for the rapid detection of 6 clinically prevalent carbapenemase genes: Klebsiella pneumoniae carbapenemase, New Delhi metallo-β-lactamase, oxacillinase, imipenem-hydrolyzing, Verona integron-encoded metallo-β-lactamase, and Guiana extended-spectrum β-lactamase. Methods The triplex assay was verified by testing genomic DNA of 6 carbapenemase-producing Klebsiella pneumoniae. It was validated with a blinded panel of 310 Enterobacteriaceae isolates, including 225 carbapenemase-producers and 85 non-producers, by direct colony triplex real-time polymerase chain reaction (PCR). The real-time PCR was performed using the ABI 7500 fast instrument (Applied Biosystems, CA, USA) and specific primers for each carbapenemase target were designed to include modified peptide-nucleic acid oligonucleotides. Results No amplification was detected among the negative samples. The result showed 100% concordance with the genotypes previously identified. The entire assay, including DNA extraction and real-time PCR, was completed within 2 hours. Conclusion The newly developed triplex real-time PCR assay was useful for the rapid, accurate and simultaneous detection of 6 carbapenemase genes in Enterobacteriaceae, suggesting its potential to allow an early decision on the appropriate treatment, management, and prevention of the spread of resistant infections in hospitals.
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등록된 정보가 없습니다.
참고문헌 (39)
참고문헌 신청
Pitout JDD / 2008 / Extended-spectrum β-lactamase-producing Enterobacteriaceae : An emerging public-health concern / Lancet Infect Dis 8(3):159~166
Falagas ME / 2007 / Pandrug-resistant Gram-negative bacteria : The dawn of the post-antibiotic era / Int J Antimicrob Agents 29(6):630~636
Davies SC / 2013 / Annual Report of the Chief Medical Officer : Infection and the rise of antimicrobial resistance / Lancet 381(9878):1606~1609
Shaikh S / 2015 / Antibiotic resistance, and extended spectrum beta-lactamases: Types, epidemiology, and treatment / Saudi J Biol Sci 22(1):90~101
Perez F / 2013 / Carbapenem-resistant Enterobacteriaceae : A menace to our most vulnerable patients / Cleve Clin J Med 80(4):225~233
Falagas ME / 2007 / Pandrug-resistant Gram-negative bacteria : The dawn of the post-antibiotic era / Int J Antimicrob Agents 29(6):630~636
Davies SC / 2013 / Annual Report of the Chief Medical Officer : Infection and the rise of antimicrobial resistance / Lancet 381(9878):1606~1609
Shaikh S / 2015 / Antibiotic resistance, and extended spectrum beta-lactamases: Types, epidemiology, and treatment / Saudi J Biol Sci 22(1):90~101
Perez F / 2013 / Carbapenem-resistant Enterobacteriaceae : A menace to our most vulnerable patients / Cleve Clin J Med 80(4):225~233
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