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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 19 - 28 (10page)
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BACKGROUND: Tissue engineering is a multidisciplinary field which attracted much attention in recent years. One of the most important issue in tissue engineering is how to obtain high cell numbers and tissue regeneration while maintaining appropriate cellular characteristics in vitro for restoring damaged or dysfunctional body tissues and organs. These demands can be achieved by the use of three dimensional (3D) dynamic cultures of cells combined with cell-adhesive micro-carriers. METHOD: In this study, human mesenchymal stem cells (hMSCs) were cultured in a wave-bioreactor system for up to 100 days, after seeding on Cultisphere-S porous gelatin micro-carriers. Cell counting was performed at the time points of 7, 12, 17, 31 days and compared to those of hMSCs cultured under static condition. Higher growth and proliferation rates was achieved in wave-type dynamic culture, when cell culture continued to day 31. A scanning electron microscope (SEM) photographs, both live and dead and MTT assays were taken to confirm the survival and distribution of cells on porous gelatin micro-carrier surfaces. The results of histological stains such as hematoxylin and eosin, Masson’s trichrome, Alcian blue and Alizarin red S also showed improved proliferation and tissue regeneration of hMSCs on porous gelatin micro-carriers. CONCLUSION: The experimental results demonstrated the effect and importance of both micro-carriers and bioreactor in hMSC expansion on cell proliferation and migration as well as extracellular matrix formation on the superficial and pore surfaces of the porous gelatin micro-carriers, and then their inter-connections, leading to tissue regeneration.
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참고문헌 (32)
참고문헌 신청
Polo-Corrales L / 2014 / Scaffold design for bone regeneration / JNanosci Nanotechnol 14:15~56
Berthiaume F / 2011 / Tissue engineering and regenerative medicine : history, progress, and challenges / Annu Rev Chem Biomol Eng 2:403~430
Sun LY / 2010 / Cell proliferation of human bone marrow mesenchymal stem cells on biodegradable microcarriers enhances in vitro differentiation potential / Cell Prolif 43:445~446
Sun LY / 2011 / Functional cells cultured on microcarriers for use in regenerative medicine research / Cell Transplant 20:49~62
van Wezel AL / 1967 / Growth of cell-strains and primary cells on microcarriers in homogeneous culture / Nature 216:64~65
Berthiaume F / 2011 / Tissue engineering and regenerative medicine : history, progress, and challenges / Annu Rev Chem Biomol Eng 2:403~430
Sun LY / 2010 / Cell proliferation of human bone marrow mesenchymal stem cells on biodegradable microcarriers enhances in vitro differentiation potential / Cell Prolif 43:445~446
Sun LY / 2011 / Functional cells cultured on microcarriers for use in regenerative medicine research / Cell Transplant 20:49~62
van Wezel AL / 1967 / Growth of cell-strains and primary cells on microcarriers in homogeneous culture / Nature 216:64~65
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