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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 660 - 669 (10page)
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Purpose: Neonatal hypoxic ischemic encephalopathy (HIE) is an essential factor underlying neonatal death and disability. Thisstudy sought to explore the role of miR-146b-5p in regulating neonatal HIE. Materials and Methods: In vitro and in vivo HIE models were established in PC12 cells and 10-day neonatal Sprague Dawley rats,respectively. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to assess miR-146b-5p expressionand inflammatory factors [interleukin (IL)-6 and tumor necrosis factor (TNF)-α] in brain lesions and PC12 cells, while enzymelinkedimmunosorbent assay was employed to detect the expression of oxidative stress factors (SOD and GSH-Px). Gain- andloss-assays of miR-146b-5p were conducted to verify its role in modulating the viability and apoptosis of PC12 cells under oxygenglucosedeprivation (OGD) treatment. Expression of TLR4, IRAK1, TRAF6, TAK1, and NF-κB were examined by qRT-PCR and/orWestern blot. Dual luciferase activity assay was conducted to identify relationships between miR-146b-5p and IRAK1. Results: In the HIE models, significant oxidative stress and inflammatory responses emerged upon upregulation of TLR4/IRAK1/TRAF6/TAK1/NF-κB signaling. Overexpression of miR-146b-5p greatly inhibited OGD-induced PC12 cell injury, inflammatoryresponses, and oxidative stress. Inhibiting miR-146b-5p, however, had the opposite effects. IRAK1 was found to be a target of miR-146b-5p, and miR-146b-5p overexpression suppressed the activation of IRAK1/TRAF6/TAK1/NF-κB signaling. Conclusion: This study demonstrated that miR-146b-5p overexpression alleviates HIE-induced neuron injury by inhibiting theIRAK1/TRAF6/TAK1/NF-κB pathway.
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참고문헌 (37)
참고문헌 신청
Li B / 2017 / Brain-immune interactions in perinatal hypoxic-ischemic brain injury / Prog Neurobiol 159:50~68
Borjini N / 2019 / Potential biomarkers for neuroinflammation and neurodegeneration at short and long term after neonatal hypoxicischemic insult in rat / J Neuroinflammation 16:194
Zhou KQ / 2019 / The role of connexin and pannexin channels in perinatal brain injury and inflammation / Front Physiol 10:141
Driscoll DJO’ / 2018 / Mild prenatal hypoxia-ischemia leads to social deficits and central and peripheral inflammation in exposed offspring / Brain Behav Immun 69:418~427
Tili E / 2013 / MicroRNAs play a central role in molecular dysfunctions linking inflammation with cancer / Immunol Rev 253:167~184
Borjini N / 2019 / Potential biomarkers for neuroinflammation and neurodegeneration at short and long term after neonatal hypoxicischemic insult in rat / J Neuroinflammation 16:194
Zhou KQ / 2019 / The role of connexin and pannexin channels in perinatal brain injury and inflammation / Front Physiol 10:141
Driscoll DJO’ / 2018 / Mild prenatal hypoxia-ischemia leads to social deficits and central and peripheral inflammation in exposed offspring / Brain Behav Immun 69:418~427
Tili E / 2013 / MicroRNAs play a central role in molecular dysfunctions linking inflammation with cancer / Immunol Rev 253:167~184
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