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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 934 - 953 (20page)
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Ciglitazone is a member of the thiazolidinedione family, and specifically binds to peroxisome proliferator-activated receptor-γ (PPARγ), thereby promoting adipocyte differentiation. We hypothesized that ciglitazone as a PPARγ ligand in the absence of an adipocyte differentiation cocktail would increase adiponectin and adipogenic gene expression in bovine satellite cells (BSC). Muscle-derived BSCs were isolated from six, 18-month-old Yanbian Yellow Cattle. The BSC were cultured for 96 h in differentiation medium containing 5 μM ciglitazone (CL), 10 μM ciglitazone (CM), or 20 μM ciglitazone (CH). Control (CON) BSC were cultured only in a differentiation medium (containing 2% horse serum). The presence of myogenin, desmin, and paired box 7 (Pax7) proteins was confirmed in the BSC by immunofluorescence staining. The CL, CM, and CH treatments produced higher concentrations of triacylglycerol and lipid droplet accumulation in myotubes than those of the CON treatment. Ciglitazone treatments significantly increased the relative expression of PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), C/EBPβ, fatty acid synthase, stearoyl-CoA desaturase, and perilipin 2. Ciglitazone treatments increased gene expression of Pax3 and Pax7 and decreased expression of myogenic differentiation-1, myogenin, myogenic regulatory factor-5, and myogenin-4 (p < 0.01). Adiponectin concentration caused by ciglitazone treatments was significantly greater than CON (p < 0.01). RNA sequencing showed that 281 differentially expressed genes (DEGs) were found in the treatments of ciglitazone. DEGs gene ontology (GO) analysis showed that the top 10 GO enrichment significantly changed the biological processes such as protein trimerization, negative regulation of cell proliferation, adipocytes differentiation, and cellular response to external stimulus. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that DEGs were involved in the p53 signaling pathway, PPAR signaling pathway, biosynthesis of amino acids, tumor necrosis factor signaling pathway, non-alcoholic fatty liver disease, PI3K-Akt signaling pathway, and Wnt signaling pathway. These results indicate that ciglitazone acts as PPARγ agonist, effectively increases the adiponectin concentration and adipogenic gene expression, and stimulates the conversion of BSC to adipocyte-like cells in the absence of adipocyte differentiation cocktail.
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참고문헌 (27)
참고문헌 신청
Karen Yeow / 2001 / Inhibition of myogenesis enables adipogenic trans-differentiation in the C2C12 myogenic cell line / FEBS Letters 506(2):157~162
N. K. Singh / 2007 / Transdifferentiation of porcine satellite cells to adipoblasts with ciglitizone1 / Journal of Animal Science 85(5):1126~1135
Xiang Z Li / 2019 / Oleic acid in the absence of a PPARγ agonist increases adipogenic gene expression in bovine muscle satellite cells1 / Journal of Animal Science 97(10):4114~4123
Lydia Teboul / 1995 / Thiazolidinediones and Fatty Acids Convert Myogenic Cells into Adipose-like Cells / Journal of Biological Chemistry 270(47):28183~28187
K.Y. Chung / 2006 / Lipid characteristics of subcutaneous adipose tissue and M. longissimus thoracis of Angus and Wagyu steers fed to US and Japanese endpoints / Meat Science 73(3):432~441
N. K. Singh / 2007 / Transdifferentiation of porcine satellite cells to adipoblasts with ciglitizone1 / Journal of Animal Science 85(5):1126~1135
Xiang Z Li / 2019 / Oleic acid in the absence of a PPARγ agonist increases adipogenic gene expression in bovine muscle satellite cells1 / Journal of Animal Science 97(10):4114~4123
Lydia Teboul / 1995 / Thiazolidinediones and Fatty Acids Convert Myogenic Cells into Adipose-like Cells / Journal of Biological Chemistry 270(47):28183~28187
K.Y. Chung / 2006 / Lipid characteristics of subcutaneous adipose tissue and M. longissimus thoracis of Angus and Wagyu steers fed to US and Japanese endpoints / Meat Science 73(3):432~441
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