지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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초록· 키워드
오류제보하기
The high-molecular-weight glutenin subunit (HMW-GS) composition of wheat is the main factor controlling gluten strength related to bread baking quality. Reported molecular markers for HMW-GS were validated and selected for improved breeding efficiency in South Korean wheat breeding programs. Sodium dodecyl sulfate polyacrylamide gel electrophoresis, lab-on-a-chip electrophoresis, sequence-tagged site (STS) markers, and Kompetitive Allele-Specific PCR were performed to re-evaluate the known HMW-GS of 14 wheat cultivars. Glu-A1b and Glu-A1c alleles were separated by the STS marker, UMN19, and KASP marker, namely Glu-Ax1/2*_SNP, at Glu-1 loci. At the Glu-B1 locus, Glu1-By8 and Glu1-By9 could be distinguished from Glu-B1b and Glu-B1c alleles by two STS markers, namely ZSBy8 and ZSBy9a, respectively. Glu1-Bx17 and Glu1-7OE could respectively be separated from non-Glu-B1i and non-Glu-B1al alleles by cauBx642 and BX7OE_866_SNP. The Glu-D1d allele, used to determine bread baking quality, could easily be distinguished from other alleles by Glu-D1d_SNP at Glu-D1 loci. Validated molecular markers in this study could therefore be used to select wheat lines for good bread baking quality in South Korean wheat breeding programs.
목차
등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
Ahmad M / 2002 / Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR-generated DNA markers / Theor Appl Genet 101:892~896
Allen AM / 2013 / Discovery and development of exome-based, co-dominant single nucleotide polymorphism markers in hexaploid wheat(Triticum aestivum L. ) / Plant Biotechnol J 11:279~295
Anderson O / 1989 / The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat / Theor Appl Genet 77:689~700
Anjum FM / 2007 / Wheat gluten: high molecular weight glutenin subunits--structure, genetics, and relation to dough elasticity / J Food Sci 72:R56~R63
Ayalew H / 2019 / Comparison of TaqMan, KASP and rhAmp SNP genotyping platforms in hexaploid wheat / PloS one 14:e0217222
Allen AM / 2013 / Discovery and development of exome-based, co-dominant single nucleotide polymorphism markers in hexaploid wheat(Triticum aestivum L. ) / Plant Biotechnol J 11:279~295
Anderson O / 1989 / The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat / Theor Appl Genet 77:689~700
Anjum FM / 2007 / Wheat gluten: high molecular weight glutenin subunits--structure, genetics, and relation to dough elasticity / J Food Sci 72:R56~R63
Ayalew H / 2019 / Comparison of TaqMan, KASP and rhAmp SNP genotyping platforms in hexaploid wheat / PloS one 14:e0217222
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