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This study is to raise the utilization of genetic resources of wheat (Triticum aestivum L.) landrace from East Asia (Korea, Japan, and China) by evaluating genetic variation related to end use quality concerned to bread making quality and early maturity, two very important characteristics of Korean wheat cropping system. To clarify the allelic variation of Glu-1 loci which decides HMW glutenin subunit composition, SDS-PAGE and DNA marker analysis were conducted by using 485 East Asian landrace wheat accessions in National Agrobiodiversity Center, RDA and NIAS genebank. And useful accessions getting full mark of glu-1 score and early maturity were selected to enhance the utilization of genetic resources to Korean wheat breeding.
In this study, wheats from China showed the distinct characteristic. Whereas Glu-A1c (null) and Glu-B1b (7+8) allele are the most frequent in Korean and Japanese accessions, Glu-A1a (2*) and Glu-B1c (7*+9) are the most in Chinese accessions. When it comes to unique composition, Glu-B1f (13+16) and Glu-D1e (2+10) subunits are only in Chinese resources. Glu-B1d (6+8), Glu-B1e (20), Glu-D1b (7+8), and Glu-D1c (4+1) subunits are only in Korean resources. The accessions from China also has high PIC value (0.53) compared to ones from Korea (0.35) and Japan (0.35). Grouping by UPGMA analysis of combination of Glu-1 allele, most accessions from Korea and Japan are in the same group, but most Chinese ones were distinguished as the distinctive group. The evaluation of bread baking quality by Glu-1 scoring system, 26 accessions got full marks. Among them, 16 accessions from China were also matured before early June, suitable to Korean cropping system. Especially, 3 accessions (K151847, K151865, K151962) had very early maturity, matured in late May. These genetic resources, having good gluten composition and early maturity, are expected to widely be used for Korean wheat breeding.
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참고문헌 (28)
참고문헌 신청
Ahn JH / 2014 / Evaluation of genetic variation at glutenin loci in Korean wheat landraces using allele-specific DNA markers / Plant Genet. Resour.-Charact.Util 12 : 353 ~ 35
Borlaug NE / 1968 / Wheat breeding and its impact on world food supply. vol.1335 / Australian Academy of Science
Cornish GB / 2006 / Gliadin and Glutenin: the unique balance of wheat quality / Am. Assoc. Cereal Chem : 243 ~ 280
Dellaporta SL / 1983 / A plant DNA minipreparation: versionⅡ / Plant Mol. Biol. Rep 1 : 19 ~ 21
D’Ovidio R / 2004 / The low-molecular-weight glutenin subunits of wheat gluten / J. Cereal Sci 39 : 321 ~ 339
Borlaug NE / 1968 / Wheat breeding and its impact on world food supply. vol.1335 / Australian Academy of Science
Cornish GB / 2006 / Gliadin and Glutenin: the unique balance of wheat quality / Am. Assoc. Cereal Chem : 243 ~ 280
Dellaporta SL / 1983 / A plant DNA minipreparation: versionⅡ / Plant Mol. Biol. Rep 1 : 19 ~ 21
D’Ovidio R / 2004 / The low-molecular-weight glutenin subunits of wheat gluten / J. Cereal Sci 39 : 321 ~ 339
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