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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 694 - 704 (11page)
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HslUV is a bacterial heat shock protein complex consisting of the AAA+ ATPase component HslU and the protease component HslV. HslV is a threonine (Thr) protease employing the N-terminal Thr residue in the mature protein as the catalytic residue. To date, HslUV from Gram-negative bacteria has been extensively studied. However, the mechanisms of action and activation of HslUV from Gram-positive bacteria, which have an additional N-terminal sequence before the catalytic Thr residue, remain to be revealed. In this study, we determined the crystal structures of HslV from the Gram-positive bacterium Staphylococcus aureus with and without HslU in the crystallization conditions. The structural comparison suggested that a structural transition to the symmetric form of HslV was triggered by ATP-bound HslU. More importantly, the additional N-terminal sequence was cleaved in the presence of HslU and ATP, exposing the Thr9 residue at the N-terminus and activating the ATP-dependent protease activity. Further biochemical studies demonstrated that the exposed N-terminal Thr residue is critical for catalysis with binding to the symmetric HslU hexamer. Since eukaryotic proteasomes have a similar additional N-terminal sequence, our results will improve our understanding of the common molecular mechanisms for the activation of proteasomes.
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참고문헌 (32)
참고문헌 신청
Baytshtok, V. / 2016 / A Structurally Dynamic Region of the HslU Intermediate Domain Controls Protein Degradation and ATP Hydrolysis / Structure 24:1766~1777
Bochtler, M. / 1997 / Crystal structure of heat shock locus V(HslV)from Escherichia coli / Proc. Natl. Acad. Sci. U. S. A 94:6070~6074
Bochtler, M. / 2000 / The structures of HsIU and the ATP-dependent protease HslU-HslV / Nature 403:800~805
Cabrita, L. D. / 2006 / A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production / BMC Biotechnol 6:12
Chuang, S. E. / 1993 / Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli / Gene 134:1~6
Bochtler, M. / 1997 / Crystal structure of heat shock locus V(HslV)from Escherichia coli / Proc. Natl. Acad. Sci. U. S. A 94:6070~6074
Bochtler, M. / 2000 / The structures of HsIU and the ATP-dependent protease HslU-HslV / Nature 403:800~805
Cabrita, L. D. / 2006 / A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production / BMC Biotechnol 6:12
Chuang, S. E. / 1993 / Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli / Gene 134:1~6
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