지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.1
- 수록면
- 205 - 212 (8page)
이용수
초록· 키워드
오류제보하기
The tumor suppressor protein p53 is stabilized by the herpes-virus-associated ubiquitin-specific protease (HAUSP), a deubiquitinating enzyme. We previously isolated and characterized a mouse orthologue of HAUSP, mHAUSP. mHAUSP cDNA consisted of 3,312 bp encodes 1,103 amino acids with a molecular weight of approximately 135 kDa containing highly conserved Cys, Asp (I), His, and Asn/Asp (II) domains. In this study, we carried out site-directed mutagenesis of 6 conserved amino acids (Cys224, Gln231, Asp296, His457, His465, and Asp482) in Cys box, QQD box, and His box. Interestingly, the conserved Gln 231 was not essential for the catalytic activity of mHAUSP. However, the other conserved amino acids were required for deubiquitinating activity of mHAUSP. We performed isopeptidase assay and confirmed that mHAUSP is able to remove ubiquitin from ubiquitinated substrates. In addition, we observed that mHAUSP induces apoptosis in HeLa cells.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Cloning and Properties of the Homologs of a Ubiquitin Specific Protease, HAUSP
한국동물학회 학술대회
2001 .01
-
Mechanism of HAUSP Inhibition by a Viral Interferon Regulatory Factor Protein 4
한국미생물학회 학술대회논문집
2013 .05
-
Conjugation and deconjugation of ubiquitin regulating the destiny of proteins
Experimental and Molecular Medicine
2003 .01
-
Structural Insights into the Interaction of the Kaposi"s Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor with the Deubiquitinase HAUSP
한국미생물학회 학술대회논문집
2012 .05
-
Conserved Amino Acid Sequence-based Semi-rational Approach for Engineering Industrial Enzymes
한국미생물학회 학술대회논문집
2013 .05
-
Substitutions for Cys-472 and His-509 at the Active Site of β-Galactosidase from Lactococcus lactis ssp. lactis 7962 Cause Large Decreases in Enzyme Activity
Journal of Microbiology and Biotechnology
2006 .01
-
Identification of Catalytic Amino Acid Residues by Chemical Modification in Dextranase
Journal of Microbiology and Biotechnology
2016 .01
-
형광항체반응(螢光抗體反應)에 의(依)한 Aspergillus spp.의 분류(分類)에 관(關)한 연구(硏究)
한국균학회지
1973 .01
-
돌돔(Oplegnathus fasciatus) somatolactin cDNA의 분석
생명과학회지
2003 .12
-
Herpesvirus-encoded Deubiquitinating Proteases and Their Roles in Regulating Immune Signaling Pathways
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2013 .12
-
The Ubiquitin-Proteasome System and F-box Proteins in Pathogenic Fungi
Mycobiology
2011 .01
-
Role of Amino Acids in Production of D-amino Acid Oxidase
The Journal of Microbiology
2001 .09
-
미생물의 보존적 유전자 탐색
생명과학회지
2005 .04
-
Critical Roles of Deubiquitinating Enzymes in the Nervous System and Neurodegenerative Disorders
Molecules and Cells
2020 .01
-
Site-directed mutagenesis of the conserved amino acid residues in levan fructotransferase from Microbacterium sp.
한국생명과학회 심포지움
2003 .05
-
In Silico Study of Human Gap Junction Beta-2 Protein by Homology Modeling
Genomics & informatics
2010 .01
-
Analysis of structural amino acid contents in chicken undergoing different cooking methods using automated amino acid analyzer
한국분석과학회 학술대회
2019 .11
-
A novel F-box protein with leucine-rich repeats affects defecation frequency and daumone response in Caenorhabditis elegans
Animal cells and systems
2012 .01
-
Chemically Induced Cellular Proteolysis: An Emerging Therapeutic Strategy for Undruggable Targets
Molecules and Cells
2018 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0