Suppression of the Epidermal Growth Factor-like Domain 7 and Inhibition of Migration and Epithelial-Mesenchymal Transition in Human Pancreatic Cancer PANC-1 Cells

Wang, Yun-Liang; Dong, Feng-Lin; Yang, Jian; Li, Zhi; Zhi, Qiao-Ming; Zhao, Xin; Yang, Yong; Li, De-Chun; Shen, Xiao-Chun; Zhou, Jin

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자료유형: 학술저널

저자정보: Wang, Yun-Liang (Department of General Surgery, the First Affiliated Hospital of Soochow University) Dong, Feng-Lin (Department of Ultrasonography, the First Affiliated Hospital of Soochow University) Yang, Jian (Department of General Surgery, the First Affiliated Hospital of Soochow University) Li, Zhi (Department of Interventional Radiology, the First Affiliated Hospital of Soochow University) Zhi, Qiao-Ming (Department of General Surgery, the First Affiliated Hospital of Soochow University) Zhao, Xin (Department of General Surgery, the First Affiliated Hospital of Soochow University) Yang, Yong (Department of General Surgery, the First Affiliated Hospital of Soochow University) Li, De-Chun (Department of General Surgery, the First Affiliated Hospital of Soochow University) Shen, Xiao-Chun (Department of Respiratory Medicine, the First Affiliated Hospital of Soochow University) Zhou, Jin (Department of General Surgery, the First Affiliated Hospital of Soochow University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제9호

발행연도: 2015.1

수록면: 4,065 - 4,069 (5page)

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Background: Epidermal growth factor-like domain multiple 7 (EGFL7), a secreted protein specifically expressed by endothelial cells during embryogenesis, recently was identified as a critical gene in tumor metastasis. Epithelial-mesenchymal transition (EMT) was found to be closely related with tumor progression. Accordingly, it is important to investigate the migration and EMT change after knock-down of EGFL7 gene expression in human pancreatic cancer cells. Materials and Methods: EGFL7 expression was firstly testified in 4 pancreatic cancer cell lines by real-time polymerase chain reaction (Real-time PCR) and western blot, and the highest expression of EGFL7 was found in PANC-1 cell line. Then, PANC-1 cells transfected with small interference RNA (siRNA) of EGFL7 using plasmid vector were named si-PANC-1, while transfected with negative control plasmid vector were called NC-PANC-1. Transwell assay was used to analyze the migration of PANC-1 cells. Real-time PCR and western blotting were used to detect the expression change of EGFL7 gene, EMT markers like E-Cadherin, N-Cadherin, Vimentin, Fibronectin and transcription factors like snail, slug in PANC-1, NCPANC-1, and si-PANC-1 cells, respectively. Results: After successful plasmid transfection, EGFL7 gene were dramatically knock-down by RNA interference in si-PANC-1 group. Meanwhile, migration ability decreased significantly, compared with PANC-1 and NC-PANC-1 group. Meanwhile, the expression of epithelial phenotype marker E-Cadherin increased and that of mesenchymal phenotype markers N-Cadherin, Vimentin, Fibronectin dramatically decreased in si-PANC-1 group, indicating a reversion of EMT. Also, transcription factors snail and slug decreased significantly after RNA interference. Conclusions: Current study suggested that highly-expressed EGFL7 promotes migration of PANC-1 cells and acts through transcription factors snail and slug to induce EMT, and further study is needed to confirm this issue.

#Pancreatic cancer #epidermal growth factor-like domain multiple 7 #epithelial-mesenchymal transition

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