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저자정보
Choi, Jae Kwon (Central Research Institute, Dr. Chung's Food Co. Ltd.) Lee, Yoon Bok (Central Research Institute, Dr. Chung's Food Co. Ltd.) Lee, Kyun Hee (Central Research Institute, Dr. Chung's Food Co. Ltd.) Im, Hae Cheon (Central Research Institute, Dr. Chung's Food Co. Ltd.) Kim, Yun Bae (College of Veterinary Medicine, Chungbuk National University) Choi, Ehn Kyoung (College of Veterinary Medicine, Chungbuk National University) Joo, Seong Soo (Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University) Jang, Su Kil (Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University) Han, Nam Soo (Department of Food Science and Technology, Chungbuk National University) Kim, Chung Ho (Department of Food and Nutrition, Seowon University)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제58권 제2호
발행연도
2015.1
수록면
117 - 124 (8page)

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The extract of white rose petals has an antioxidant effect and can be used to treat allergic disease. The purpose of this study was to identify optimal conditions for extracting antioxidative compounds from white rose petals with 2,2-diphenyl-1-picrylhydrazyl scavenging activities. A response surface methodology based on a central composite design was used to investigate the effects of three independent variables: ethanol concentration ($X_1$), extraction temperature ($X_2$), and extraction time ($X_3$). The estimated optimal conditions for obtaining phenolic compounds with antioxidant activities were as follows: ethanol concentration of 42% ($X_1$), extraction time of 80 min ($X_3$), and extraction temperature of $75^{\circ}C$ ($X_2$). The estimated optimal conditions for obtaining flavonoid compounds with antioxidant effects were an ethanol concentration of 41% ($X_1$), extraction time of 119 min ($X_3$), and an extraction temperature of $75^{\circ}C$ ($X_2$). Under these conditions, predicted response values for the phenolic and flavonoid contents were 243.5 mg gallic acid equivalent/g dry mass and 19.93 mg catechin equivalent (CE)/g dry mass, respectively.

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