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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Ka-Jeong (Food Safety Research Division, National Fisheries Research & Development Institute) Park, Hee-Yeon (Biotechnology Research Institute, National Fisheries Research & Development Institute) Kim, Yeon-Kye (Biotechnology Research Institute, National Fisheries Research & Development Institute) Park, Jin-Il (Biotechnology Research Institute, National Fisheries Research & Development Institute) Yoon, Ho-Dong (Food Safety Research Division, National Fisheries Research & Development Institute)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제52권 제3호
발행연도
2009.1
수록면
221 - 226 (6page)

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The industrial use of bovine and porcine collagen has been expanded to include foods, cosmetics, and biomaterials, However, due to outbreaks of bovine spongiform encephalopathy and foot and mouth disease, new, alternative, safe sources of collagen are being sought. In this study, pepsin-solubilized collagen (PSC) was isolated from the tissue of a starfish (Asterias amurensis) and shown to consist of (${\alpha}1)_2{\alpha}2$ heterotrimer, which is similar to calf skin type I collagen. However, the PSC was denaturated at 24.7$^{\circ}C$, which is about 12$^{\circ}C$ lower than mammalian collagen. Immunoblotting assay using polyclonal anti-type I collagen antibody revealed that the starfish collagen contained similar affinity motifs. In XTT assay, PSC suspension had cell growth activity and no showed no cytotoxicity.

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