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논문 기본 정보

자료유형
학술저널
저자정보
Hwang, In-Wook (Department of Food Science and Nutrition, Dong-A University) Kim, Bo-Min (School of Food Science and Biotechnology, Kyungpook National University) Kim, Young-Chan (Korea Food Research Institute) Lee, Sang-Han (School of Food Science and Biotechnology, Kyungpook National University) Chung, Shin-Kyo (School of Food Science and Biotechnology, Kyungpook National University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제61권 제2호
발행연도
2018.1
수록면
235 - 242 (8page)

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In this study, the high-pressure steaming and enzymatic pre-treatment (SET) was used to improve ${\beta}$-glucan extraction from Ganoderma lucidum (G. lucidum), an oriental medicinal mushroom. Response surface methodology and central composite design were used to determine the optimum pre-treatment conditions: high-pressure steaming, enzymatic hydrolysis, and Viscozyme L concentrations. The optimal conditions were 15.51 min for high-pressure steaming, 0.84 g/100 mL of Viscozyme L, and 4.16 h for hydrolysis. The predicted ${\beta}$-glucan content in G. lucidum extract at optimal conditions, approximately twofold (8.05 g/100 g) of the control treatment value, was consistent with the empirical value. The total sugar and protein contents through SET were higher than those values of the control treatment. The cell migration assay showed that SET-processed G. lucidum extracts significantly suppressed B16F10 murine melanoma cell growth. SET process using Viscozyme L could be utilized for ${\beta}$-glucan extraction from G. lucidum to develop the functional food.

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