Exogenous p53 Upregulated Modulator of Apoptosis (PUMA) Decreases Growth of Lung Cancer A549 Cells

Liu, Chun-Ju; Zhang, Xia-Li; Luo, Da-Ya; Zhu, Wei-Feng; Wan, Hui-Fang; Yang, Jun-Ping; Yang, Xiao-Jun; Wan, Fu-Sheng

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자료유형: 학술저널

저자정보: Liu, Chun-Ju (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University) Zhang, Xia-Li (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University) Luo, Da-Ya (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University) Zhu, Wei-Feng (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University) Wan, Hui-Fang (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University) Yang, Jun-Ping (Clinical Laboratory of Jiangxi University of Traditional Chinese Medicine) Yang, Xiao-Jun (Clinical Laboratory of Jiangxi University of Traditional Chinese Medicine) Wan, Fu-Sheng (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제2호

발행연도: 2015.1

수록면: 741 - 746 (6page)

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Purpose: To investigate the influence of exogenous p53 upregulated modulator of apoptosis (PUMA) expression on cell proliferation and apoptosis in human non-small cell lung cancer A549 cells and transplanted tumor cell growth in nude mice. Materials and Methods: A549 cells were divided into the following groups: control, non-carrier (NC), PUMA (transfected with pCEP4-(HA) 2-PUMA plasmid), DDP ($10{\mu}g/mL$ cisplatin treatment) and PUMA+DDP (transfected with pCEP4-(HA)2-PUMA plasmid and $10{\mu}g/mL$ cisplatin treatment). The MTT method was used to detect the cell survival rate. Cell apoptosis rates were measured by flow cytometry, and PUMA, Bax and Bcl-2 protein expression levels were measured by Western blotting. Results: Compared to the control group, the PUMA, DDP and PUMA+DDP groups all had significantly decreased A549 cell proliferation (p<0.01), with the largest reduction in the PUMA+DDP group. Conversely, the apoptosis rates of the three groups were significantly increased (P<0.01), and the PUMA and DDP treatments were synergistic. Moreover, Bax protein levels significantly increased (p<0.01), while Bcl-2 protein levels significantly decreased (p<0.01). Finally, both the volume and the weights of transplanted tumors were significantly reduced (p<0.01), and the inhibition ratio of the PUMA+DDP group was significantly higher than in the single DDP or PUMA groups. Conclusions: Exogenous PUMA effectively inhibited lung cancer A549 cell proliferation and transplanted tumor growth by increasing Bax protein levels and reducing Bcl-2 protein levels.

#p53 upregulated modulator of apoptosis #non-small cell lung cancer #apoptosis #tumor transplant

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Liu, Chun-Ju

소속기관 Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University