Anthraquinone Glycoside Aloin Induces Osteogenic Initiation of MC3T3-E1 Cells: Involvement of MAPK Mediated Wnt and Bmp Signaling

Pengjam, Yutthana; Madhyastha, Harishkumar; Madhyastha, Radha; Yamaguchi, Yuya; Nakajima, Yuichi; Maruyama, Masugi

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저자정보: Pengjam, Yutthana (Department of Applied Physiology, Faculty of Medicine, University of Miyazaki) Madhyastha, Harishkumar (Department of Applied Physiology, Faculty of Medicine, University of Miyazaki) Madhyastha, Radha (Department of Applied Physiology, Faculty of Medicine, University of Miyazaki) Yamaguchi, Yuya (Department of Applied Physiology, Faculty of Medicine, University of Miyazaki) Nakajima, Yuichi (Department of Applied Physiology, Faculty of Medicine, University of Miyazaki) Maruyama, Masugi (Department of Applied Physiology, Faculty of Medicine, University of Miyazaki)

저널정보: 한국응용약물학회 Biomolecules & Therapeutics(구 응용약물학회지) Biomolecules & therapeutics 제24권 제2호

발행연도: 2016.1

수록면: 123 - 131 (9page)

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Osteoporosis is a bone pathology leading to increased fracture risk and challenging the quality of life. The aim of this study was to evaluate the effect of an anthraquinone glycoside, aloin, on osteogenic induction of MC3T3-E1 cells. Aloin increased alkaline phosphatase (ALP) activity, an early differentiation marker of osteoblasts. Aloin also increased the ALP activity in adult human adipose-derived stem cells (hADSC), indicating that the action of aloin was not cell-type specific. Alizarin red S staining revealed a significant amount of calcium deposition in cells treated with aloin. Aloin enhanced the expression of osteoblast differentiation genes, Bmp-2, Runx2 and collagen 1a, in a dose-dependent manner. Western blot analysis revealed that noggin and inhibitors of p38 MAPK and SAPK/JNK signals attenuated aloin-promoted expressions of Bmp-2 and Runx2 proteins. siRNA mediated blocking of Wnt-5a signaling pathway also annulled the influence of aloin, indicating Wnt-5a dependent activity. Inhibition of the different signal pathways abrogated the influence of aloin on ALP activity, confirming that aloin induced MC3T3-E1 cells into osteoblasts through MAPK mediated Wnt and Bmp signaling pathway.

#Aloin #MC3T3-E1 cell #MAPK #Wnt #Bmp signaling

참고문헌 (35)

참고문헌 신청

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Bennett, C.N / 2005 / Regulation of osteoblastogenesis and bone mass by Wnt10b / Proc. Natl. Acad. Sci. U.S.A 102:3324~3329

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