지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Background: Many histone deacetylase (HDAC) inhibitors are well recognized as potential anti-cancer drugs. Inhibition of HDACs induces temporal transcription or epigenetic control, thus regulating many different biological responses. Here, we investigated the osteogenic effect of the HDAC inhibitor suberoylanilide hydroxamic acid (SAHA; vorinostat).
Methods: The effects of SAHA on osteoblast differentiation were examined in the 6XOSE-Luc reporter assay for determination of runt-related transcription factor 2 (Runx2) activity and alkaline phosphatase (ALP) activity and in an immunoprecipitation assay to determine the Runx2 acetylation state. The osteogenic activity of SAHA in vivo was studied in and receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoporotic mouse model. Results: SAHA increased the transcriptional activity of Runx2 in a dose-dependent manner in the 6XOSE-Luc reporter assay. SAHA by itself was unable to induce ALP activity; however, SAHA enhanced ALP activity induced by bone morphogenetic protein-2 (BMP-2). The degree of acetylation of Runx2 was increased with SAHA treatment, which suggests that the increase in Runx2 transcriptional activity might be dependent on stabilization by acetylation. Also, SAHA successfully reversed soluble RA- NKL-induced osteoporotic bone loss. Conclusions: Our study shows an intriguing osteogenic potential of SAHA in a BMP-2-dependent manner and suggests that SAHA could be used at lower doses along with BMP-2 to treat osteoporosis.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Bolden JE / 2006 / Anticancer activities of histone deacetylase inhibitors / Nat Rev Drug Discov 5 : 769 ~ 784
Bose P / 2014 / Histone deacetylase inhibitor(HDACI)mechanisms of action : emerging insights / Pharmacol Ther 143 : 323 ~ 336
Cho HH / 2005 / Induction of osteogenic differentiation of human mesenchymal stem cells by histone deacetylase inhibitors / J Cell Biochem 96 : 533 ~ 542
Cohen MM Jr. / 2006 / The new bone biology: pathologic, molecular, and clinical correlates / Am J Med Genet A 140 : 2646 ~ 2706
Dudakovic A / 2013 / Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces Akt phosphorylation / J Biol Chem 288 : 2
Bose P / 2014 / Histone deacetylase inhibitor(HDACI)mechanisms of action : emerging insights / Pharmacol Ther 143 : 323 ~ 336
Cho HH / 2005 / Induction of osteogenic differentiation of human mesenchymal stem cells by histone deacetylase inhibitors / J Cell Biochem 96 : 533 ~ 542
Cohen MM Jr. / 2006 / The new bone biology: pathologic, molecular, and clinical correlates / Am J Med Genet A 140 : 2646 ~ 2706
Dudakovic A / 2013 / Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces Akt phosphorylation / J Biol Chem 288 : 2
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
PD-L1 Aptamer-functionalized Liposome Containing SAHA for Anti-lung Cancer Immunotherapy
대한의생명과학회지
2024 .06
-
Induction of Apoptosis in Intestinal Toxicity to a Histone Deacetylase Inhibitor in a Phase I Study with Pelvic Radiotherapy
Cancer Research and Treatment
2017 .01
-
PD-L1 Targeted Immunoliposomes with PD-L1 siRNA and HDAC Inhibitor for Anti-Lung Cancer Immunotherapy
대한의생명과학회지
2022 .12
-
Growth-suppressive effect of Suberoylanilide hydroxamic acid in Human Oral Cancer through ERK pathway
한국실험동물학회 학술발표대회 논문집
2015 .08
-
MicroRNA-181a-5p Curbs Osteogenic Differentiation and Bone Formation Partially Through Impairing Runx1-Dependent Inhibition of AIF-1 Transcription
Endocrinology and Metabolism
2023 .02
-
Comparative analysis of protein profiles between wild-type and Runx3 null mouse embryonic stem cells lines
Journal of Biomedical and Translational Research
2020 .01
-
Roles of Histone Deacetylase 4 in the Inflammatory and Metabolic Processes
Diabetes and Metabolism Journal
2024 .05
-
Sulforaphane Ameliorates Diabetes-Induced Renal Fibrosis through Epigenetic Up-Regulation of BMP-7
Diabetes and Metabolism Journal
2021 .11
-
HDAC and HDAC Inhibitor: From Cancer to Cardiovascular Diseases
전남의대학술지
2016 .01
-
Histone Deacetylase 9: Its Role in the Pathogenesis of Diabetes and Other Chronic Diseases
Diabetes and Metabolism Journal
2020 .01
-
The First Case of Acute Myeloid Leukemia With a Novel Five-way Variant Translocation of RUNX1–RUNX1T1
Annals of Laboratory Medicine
2023 .05
-
Discovery and characterization of berberine derivatives as stimulators of osteoblast differentiation
International Journal of Oral Biology
2019 .01
-
Roles of RUNX1 and PU.1 in CCR3 Transcription
Immune Network
2016 .06
-
Anthraquinone Glycoside Aloin Induces Osteogenic Initiation of MC3T3-E1 Cells: Involvement of MAPK Mediated Wnt and Bmp Signaling
Biomolecules & Therapeutics(구 응용약물학회지)
2016 .01
-
HDAC11 is involved in myogenic differentiation through its enzymatic activity
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Osteogenic potential of mesenchymal stem cells by BMP-2 plasmid DNA introduction
대한치과재료학회 학술대회
2020 .10
-
Epigenetic inactivation of RUNX3 in colorectal cancer
Annals of Surgical Treatment and Research
2018 .03
-
Histone Deacetylase-3 Modification of MicroRNA-31 Promotes Cell Proliferation and Aerobic Glycolysis in Breast Cancer and Is Predictive of Poor Prognosis
Journal of Breast Cancer
2018 .01
-
In-Silico Analysis of Chromatin Modifiers and Profiling of Histone Deacetylases (HDAC’s) in Human Oral Cancer
전남의대학술지
2021 .09
-
Stepwise verification of bone regeneration using recombinant human bone morphogenetic protein-2 in rat fibula model
대한구강악안면외과학회지
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0