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논문 기본 정보

자료유형
학술저널
저자정보
Lim, Young-Shin (College of Pharmacy, Chonnam National University) Kang, Bok-Yun (College of Pharmacy, Chonnam National University) Kim, Eui-Jin (College of Pharmacy, Chonnam National University) Kim, Seung-Hyun (College of Pharmacy, Chonnam National University) Hwang, Seung-Yong (Department of biochemistry and Molecular Biology, Hanyang University) Kim, Kyeong-Man (College of Pharmacy, chonnam National University) Kim, Tae-Sung (College of Pharmacy, Institute of Biotechnology, Chonnam National University)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제21권 제5호
발행연도
1998.1
수록면
537 - 542 (6page)

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to more effectively drive immune responses toward antigen-specific T helper type 2 (Th2) cell-mediated responses, we constructed a mammalian expression vetor (oPVA/IL4) carrying a fused gene in which the ovalbumin (OVA) cDNA was covalently linked to murine interleukin-4 (IL-4) cDNA. A biologically active OVA/IL4 DNA, as demonstrated by Wes tern blotting and cytokine bioassay. In tramuscular injection of BALB/c mice with the pOVA/IL4 DNA increased both the production of OVA-specific IL-4 by CD$4^{+}$ T cells and the ratio of anti-OVA lgG1 to anti-OVA lgG2a isotypes, while the injection with the pOVA DNA alone, or with the mixture of the pOVA and pIL4 DNA did no or little increase. furthermore, the OVA-specific, Th2 cell-mediated immune responses were significantly enhanced by multiple injections with the pOVA/IL4 DNA. These studies indicate that the direct linkage of an OVA gene to an IL-4 gene in the expression plasmid confines the effects of IL-4 to the OVA-specific cells, efficiently driving the immune response toward OVA-specific, Th2 cell-mediated responses.

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