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The Experimental Study on the Immuno-regulatory effect of Notopterygii Rhizoma Herbal-acupuncture at Pyesu(BL13) on OVA-induced asthma in mice
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폐유(肺兪) 강활약침(羌活藥鍼)이 OVA-induced Asthma Mouse Model의 면역조절(免疫調節)에 미치는 영향

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Type
Academic journal
Author
Jang, Suk-Geun (대전대학교 한의과대학 침구학교실) Hong, Kwon-Eui (대전대학교 한의과대학 침구학교실) Lee, Byung-Ryul (대전대학교 한의과대학 침구학교실)
Journal
경락경혈학회 Korean Journal of Acupuncture Korean journal of acupuncture 제22권 제2호 KCI Accredited Journals
Published
2005.1
Pages
107 - 125 (19page)

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The Experimental Study on the Immuno-regulatory effect of Notopterygii Rhizoma Herbal-acupuncture at Pyesu(BL13) on OVA-induced asthma in mice
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Objectives : The aim of this study was to investigate the asthma-suppressive and immuno-regulatory effect of NR-HA(Notopterygii Rhizoma Herbal-acupuncture) at Pyesu(BL13) on OVA(ovalbumin)-induced asthma in mice. Methods : C57BL/6 mice out of all the experimental sloops, except the Normal group and the NR-HA group, were sensitized and challenged with OVA. The mice in the NR-HA group and the OVA-NR-HA group were treated with NR-HA(1%) at Pyesu(BL13). The mice in the OVA-Saline group were injected with saline at Pyesu(BL13). The mice in the OVA-Needle-prick group were treated with a single prick with an injection needle at Pyesu(BL13). NR-HA, saline injection and needle prick were administered for 8 weeks, three times a week Results : in vitro 1. The populations of granulocytes, $CD3e^-/CCR3^+$cells, $CD69^+/CD3e^+$ cells, $CD4^+\;cells\;and\;CD23^+/B220^+$ cells in the OVA-induced asthmatic mouse lungs decreased significantly by NR-HAS(Notopterygii Rhizoma Herbal-acupuncture solution). 2. The lung weight and total cells in lung of the OVA-NR-HA group decreased significantly compared with those of the OVA-Control group. 3. Total leukocytes and eosinophils in BALF of the OVA-NR-HA group decreas ed significantly compared with those of the OVA-Control group. 4. The collagen accumulation in the lung sections of the OVA-NR-HA group decreased significantly compared with that of the OVA-control group. 5. The concentrations of IL-4, IL-5, IL-13, IgE in BALF and serum of the OVA-NR-HA group decreased significantly compared with those of the OVA- Control group. 6. The numbers of $Gr-1^+/CD11b^+,\;CCR3^+,\;CD3e^+, \;CD19^+,\;CD3e^+/CD69^+$ cells in the OVA-NR-HA group decreased significantly compared with those of the OVA-Control group. 7. The mRNA expressions of $TNF-{\alpha}$, IL-5, IL-4 and IL-13 in lung of the OVA-NR-HA group decreased significantly compared with those of the OVA- Control group. 8. The NR-HA group did not show my considerable difference from the Normal group. The OVA-saline group and the OVA-Needle prick group showed suppressive effects on OVA-induced asthma however they were not statistically significant. Conclusion : These results suggest that NR-HA at Pyesu(BL13) is considered to be effective in treating asthma and to be put to practical use in the future asthma clinic.
#Notopterygii Rhizoma #Herbal-acupuncture #BL13 #OVA #ovalbumin)-induced asthma

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