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Pholiota adiposa and its Related Species Collected from the Wild Forestry
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야생에서 채집된 검은비늘버섯(Pholiota adiposa)균에 관한 연구

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Type
Academic journal
Author
Lee, Sang-Sun (한국교원대학교 대학원 생물교육학과) Kim, Mi-Hye (한국교원대학교 대학원 생물교육학과) Chang, Hu-Bong (충북 농촌진흥원 균이계) Shin, Chun-Sik (충북 농촌진흥원 균이계) Lee, Min-Woong (동국대학교 응용미생물학과)
Journal
Korean Society of Mycology 한국균학회지 한국균학회지 제26권 제4호 KCI Accredited Journals
Published
1998.1
Pages
574 - 582 (9page)

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Pholiota adiposa and its Related Species Collected from the Wild Forestry
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Five basidiocarps of Pholiota species have been collected from the areas of BubJu Temple for last two years, and identified to those of P. adiposa or Pholiota species. The taxonomy of these basidiocarps with the morphological aspects was compared with the analysis obtained from the polymorphisms of PCR-RAPD bands made after reacted with the random primers. The polymorphic variations were observed within the species of the basidiocarps, but not between genomic DNA's of the mycelia obtained and the basidiocarps. Several different bands made from the primers (28 and 36) in PCR-RAPD reactions were identified within the genus of Pholiota and speculated to be specific for the individual basidiocarp of P. adiposa collected. The primers employed here were considered to be very useful for distinguishing the individual isolates or basidiocarps collected from the fields. Also, the basidiospores were obtained from the sporeprints of the above basidiocarps as a simple agar and confirmed with observations of clamp connection under microscopes. The matings of them indicated the 'tetrapolar' type, being different from the 'bipolar' type reported by Japanese basidiocarps of P. adiposa or P. nameko. Based on our work, the edible fungi collected were speculated to be a new breeding resource for those of Pholiota commercialized in Japan.
#Breeding #Dikaryon #PCR-RAPD #Pholiota adiposa #Random primer #Tetrapolar

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