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논문 기본 정보

자료유형
학술저널
저자정보
이주현 (경희대학교 치과대학 구강악안면방사선학 교실) 황의환 (경희대학교 치과대학 구강악안면방사선학 교실) 이상래 (경희대학교 치과대학 구강악안면방사선학 교실)
저널정보
대한영상치의학회 치과방사선 치과방사선 제27권 제2호
발행연도
1997.1
수록면
91 - 103 (13page)

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The purpose of this study was to aid in the prediction of tumor cell tolerance to radiotherapy and/or chemotherapy. For this study, cell surviving curves were obtained for human epidermoid carcinoma A-253 cell line using semiautomated MTT assay. 2,4,6,8,10 Gy were irradiated at a dose rate of 210 cGy/min using <SUP>60</SUP>Co Irradiator ALDORADO 8. After irradiation, A-253 cell lines(2&times;10&#8308;cells/mil were exposed to bleomycin or cisplatin for 1 hour. The viable cells were determined for each radiation dose with/without 2 /lg/mi of drug at the 3rd day. And they were compared to control values. The results were obtained as follows : 1. The surviving curve with gentle slope was obtained after irradiation of 2, 4, 6, 8, 10 Gy on A-253 cell line. 2. The cytotoxicity of bleomycin or cisplatin at the concentration of 2&#13197;/ml was great on A-253 cell line. But, there was no significant difference between the cytotoxicity of bleomycin and that of cisplatin. 3. There were significant differences of surviving fractions after irradiation with 2&#13197;/mi of bleomycin compared with irradiation only on A-253 cell line. 4. There were significant differences of surviving fractions after irradiation with 2&#13197;/ml of cisplatin compared with irradiation only on A-253 cell line. 5. There were no significant differences of surviving fractions between the groups of irradiation with bleomycin and the groups of irradiation with cisplatin on A-253 cell line.

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