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논문 기본 정보

자료유형
학술저널
저자정보
한원정 (서울대학교 치과대학 구강악안면방사선학 교실 및 치학연구소) 허민석 (서울대학교 치과대학 구강악안면방사선학 교실 및 치학연구소) 이삼선 (서울대학교 치과대학 구강악안면방사선학 교실 및 치학연구소) 최순철 (서울대학교 치과대학 구강악안면방사선학 교실 및 치학연구소) 박태원 (서울대학교 치과대학 구강악안면방사선학 교실 및 치학연구소)
저널정보
대한영상치의학회 대한구강악안면방사선학회지 대한구강악안면방사선학회지 제30권 제1호
발행연도
2000.1
수록면
71 - 79 (9page)

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Purpose : This study was aimed to evaluate the cell cycle arrest and apoptosis induction after irradiation and epidermal growth factor (EGF) treatment in three human epithelial tumor cell lines (A431, Siha, KB). Materials and Methods: Single irradiation of 2, 5 and 10 Gy was done on three cell lines with 5.38 Gy/min dose rate using Cs-137 irradiator at room temperature. Also, EGF of 10 ng/ml was added immediately after 10 Gy irradiation. Cell growth was evaluated by counting the living cell number using a hemocytometer at 1 day, 2 days, 3 days, 4 days and 5 days after irradiation. Cell cycle arrest and apoptosis induction were assayed with the flow cytometry at 8 hours, 12 hours, 1 day, 2 days, 3 days, 4 days and 5 days after irradiation. Results : Growth of irradiated three cell lines were inhibited in proportion to radiation dose. EGF treatment after irradiation showed various results according to cell lines. On all cell lines, G2 arrest was detected after 8 hours and maximized after 12 hours or 1 day. Amount of G2 arrest was positively dose dependent. However, EGF showed no significant change on G2 arrest. G2 arrest was recovered with time at 2 Gy and 5 Gy irradiation. However, at 10 Gy irradiation, G2 arrest was continued. Apoptosis was detected at 10 Gy irradiation. On EGF treated group after irradiation, A431 and Siha cell lines showed slightly increased apoptosis but there was no statistically significant difference. KB cell line showed no marked change of apoptosis induction. Conclusion : Irradiation effects on cell cycle arrest and apoptosis induction in three human epithelial tumor cell lines, however epidermal growth factor doesn't effect on.

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