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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제5호
- 발행연도
- 2020.1
- 수록면
- 726 - 732 (7page)
이용수
초록· 키워드
오류제보하기
Indonesian kefir grains are potential sources of lactic acid bacteria (LAB) that may act as probiotics with specific functional properties. In this study we explored the potential of the probiotic and cholesterol-lowering effect of LAB isolated from Indonesian kefir grains obtained from Bogor, Bandung, Jakarta, and Yogyakarta. The results revealed that 10 isolates showed considerable survivability at low pH and bile salt with total cell reduction of ~3 log colony-forming units per milliliter after exposure to pH 2.5 and 0.5% (w/v) bile salt for 1 and 3 h, respectively. All strains exhibited strong antimicrobial activities against pathogenic bacteria and were sensitive to a wide spectrum of antibiotics but exhibited weak bile salt hydrolase activity. Identification based on 16S RNA suggested that nine isolates were Lactobacillus kefiri and one was Lactobacillus rhamnosus. The ability of the isolates to reduce cholesterol from the media varied, ranging from 22.08% to 68.75% with the highest reduction shown by L. kefiri JK17. The ability to remove cholesterol from the media decreased greatly in resting and dead cells, ranging from 14.58% to 22.08% in resting cells and from 7.89% to 18.17% in dead cells. It can be concluded that Indonesian kefir grains contain LAB potentially acting as probiotics capable of reducing cholesterol. The cholesterol-lowering effect especially occurs when the cells are metabolically active.
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참고문헌 (40)
참고문헌 신청
Hertzler SR / 2003 / Kefir improves lactose digestion and tolerance in adults with lactose maldigestion / J. Am. Diet. Assoc 103:582~587
김동현 / 2016 / Antimicrobial Activity of Kefir against Various Food Pathogens and Spoilage Bacteria / 한국축산식품학회지 36(6):787~790
Ozcan A / 2009 / Effect of kefir on the oxidative stress due to lead in rats / J. Appl. Anim. Res 35:91~93
Liu JR / 2006 / Hypocholesterolaemic effects of milk kefir and soyamilk kefir in cholesterol-fed hamsters / Br. J. Nutr 95:939~946
Hadisaputro S / 2012 / The effects of oral plain kefir supplementation on proinflammatory cytokine properties of the hyperglycemia Wistar rats induced by streptozotocin / Acta Med. Indones 44:100~104
김동현 / 2016 / Antimicrobial Activity of Kefir against Various Food Pathogens and Spoilage Bacteria / 한국축산식품학회지 36(6):787~790
Ozcan A / 2009 / Effect of kefir on the oxidative stress due to lead in rats / J. Appl. Anim. Res 35:91~93
Liu JR / 2006 / Hypocholesterolaemic effects of milk kefir and soyamilk kefir in cholesterol-fed hamsters / Br. J. Nutr 95:939~946
Hadisaputro S / 2012 / The effects of oral plain kefir supplementation on proinflammatory cytokine properties of the hyperglycemia Wistar rats induced by streptozotocin / Acta Med. Indones 44:100~104
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