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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제12호
- 발행연도
- 2015.1
- 수록면
- 2,100 - 2,105 (6page)
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초록· 키워드
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Probiotic bacteria must have not only tolerance against bile salt but also no genes for antibiotic resistance. Leuconostoc citreum is a dominant lactic acid bacterium in various fermented foods, but it is not regarded as a probiotic because it lacks bile salt resistance. Therefore, we aimed to construct a bile salt-resistant L. citreum strain by transforming it with a bile salt hydrolase gene (bsh). We obtained the 1,001 bp bsh gene from the chromosomal DNA of Lactobacillus plantarum and subcloned it into the pCB4170 vector under a constitutive P710 promoter. The resulting vector, pCB4170BSH was transformed into L. citreum CB2567 by electroporation, and bile saltresistant transformants were selected. Upon incubation with glycodeoxycholic acid sodium salt (GDCA), the L. citreum transformants grew and formed colonies, successfully transcribed the bsh gene, and expressed the BSH enzyme. The recombinant strain grew in up to 0.3% (w/v) GDCA, conditions unsuitable for the host strain. In in vitro digestion conditions of 10 mM bile salt, the transformant was over 67.6% viable, whereas only 0.8% of the host strain survived.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Allison G / 1996 / Functional analysis of the gene encoding immunity to lactacin F, lafI, and its use as a Lactobacillus-specific, food-grade genetic marker / Appl. Environ.
Bohmer N / 2013 / Recombinant expression, purification and characterisation of the native glutamate racemase from Lactobacillus plantarum NC8 / Protein Expr. Purif 88 : 54 ~
Budde BB / 2003 / Leuconostoc carnosum 4010 has the potential for use as a protective culture for vacuum-packed meats: culture isolation, bacteriocin identification, and meat
Cho SK / 2015 / Development of food-grade vector for constitutive and inducible gene expression in Leuconostoc citreum / 박사 / Chungbuk National University
Dashkevicz MP / 1989 / Development of a differential medium for bile salt hydrolase-active Lactobacillus spp / Appl. Environ. Microbiol 55 : 11 ~ 16
Bohmer N / 2013 / Recombinant expression, purification and characterisation of the native glutamate racemase from Lactobacillus plantarum NC8 / Protein Expr. Purif 88 : 54 ~
Budde BB / 2003 / Leuconostoc carnosum 4010 has the potential for use as a protective culture for vacuum-packed meats: culture isolation, bacteriocin identification, and meat
Cho SK / 2015 / Development of food-grade vector for constitutive and inducible gene expression in Leuconostoc citreum / 박사 / Chungbuk National University
Dashkevicz MP / 1989 / Development of a differential medium for bile salt hydrolase-active Lactobacillus spp / Appl. Environ. Microbiol 55 : 11 ~ 16
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