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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제12호
- 발행연도
- 2019.1
- 수록면
- 1,938 - 1,946 (9page)
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Isomaltooligosaccharides (IMOs) have good prebiotic effects, and long IMOs (LIMOs) with a degree of polymerization (DP) of 7 or above show improved effects. However, they are not yet commercially available, and require costly enzymes and processes for production. The Nterminal region of the thermostable Thermoanaerobacter thermocopriae cycloisomaltooligosaccharide glucanotransferase (TtCITase) shows cyclic isomaltooligosaccharide (CI)-producing activity owing to a catalytic domain of glycoside hydrolase (GH) family 66 and carbohydrate-binding module (CBM) 35. In the present study, we elucidated the activity of the C-terminal region of TtCITase (TtCITase-C; Met740–Phe1,559), including a CBM35-like region and the GH family 15 domain. The domain was successfully cloned, expressed, and purified as a single protein with a molecular mass of 115 kDa. TtCITase-C exhibited optimal activity at 40°C and pH 5.5, and retained 100% activity at pH 5.5 after 18-h incubation. TtCITase-C synthesized α-1,6 glucosyl products with over seven degrees of polymerization (DP) by an α-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates. These results indicate that TtCITase-C could be used for the production of α-1,6 glucosyl oligosaccharides with over DP7 (LIMOs) in a more cost-effective manner, without requiring cyclodextran.
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참고문헌 (26)
참고문헌 신청
Gibson GR / 1995 / Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics / J, Nutr 125:1401~1412
Crittenden RG / 1996 / Production, properties and applications of food-grade oligosaccharides / Trends Food Sci, Technol 7:353~361
Kim Y-M / 2009 / Construction of a fusion enzyme of dextransucrase and dextranase: application for one-step synthesis of isomaltooligosaccharides / Enzyme Microb, Technol 44:159~164
Sadahiro J / 2015 / Extracellular and cell-associated forms of Gluconobacter oxydans dextran dextrinase change their localization depending on the cell growth / Biochem, Biophys. Res. Commun 456:500~505
Debnam ES / 1998 / Acute and chronic exposure of rat intestinal mucosa to dextran promotes SGLT1-mediated glucose transport / Eur, J. Clin. Invest 28:651~658
Crittenden RG / 1996 / Production, properties and applications of food-grade oligosaccharides / Trends Food Sci, Technol 7:353~361
Kim Y-M / 2009 / Construction of a fusion enzyme of dextransucrase and dextranase: application for one-step synthesis of isomaltooligosaccharides / Enzyme Microb, Technol 44:159~164
Sadahiro J / 2015 / Extracellular and cell-associated forms of Gluconobacter oxydans dextran dextrinase change their localization depending on the cell growth / Biochem, Biophys. Res. Commun 456:500~505
Debnam ES / 1998 / Acute and chronic exposure of rat intestinal mucosa to dextran promotes SGLT1-mediated glucose transport / Eur, J. Clin. Invest 28:651~658
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