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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제6호
- 발행연도
- 2015.1
- 수록면
- 880 - 886 (7page)
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Bacterial heme was produced from a genetic-engineered Escherichia coli via the porphyrin pathway and it was useful as an iron resource for animal feed. The amount of the E. colisynthesized heme, however, was only few milligrams in a culture broth and it was not enough for industrial applications. To analyze heme biosynthetic pathways, an engineered E. coli artificially overexpressing ALA synthase (hemA from Rhodobacter sphaeroides) and pantothenate kinase (coaA gene from self geneome) was constructed as a bacterial heme-producing strain, and both the transcription levels of pathway genes and the intermediates concentrations were determined from batch and continuous cultures. Transcription levels of the pathway genes were not significantly changed among the tested conditions. Intracellular intermediate concentrations indicated that aminolevulinic acid (ALA) and coenzyme A (CoA) were enhanced by the hemA-coaA co-expression. Intracellular coproporphyrinogen I and protoporphyrin IX accumulation suggested that the bottleneck steps in the heme biosynthetic pathway could be the spontaneous conversion of HMB to coproporphyrinogen I and the limited conversion of protoporphyrin IX to heme, respectively. A strategy to increase the conversion of ALA to heme is discussed based on the results.
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참고문헌 (12)
참고문헌 신청
Franken AC / 2011 / Heme biosynthesis and its regulation: towards understanding and improvement of heme biosynthesis in filamentous fungi / Appl. Microbiol. Biotechnol 91 : 4
Lee HJ / 2013 / Estimation of phosphoenolpyruvate carboxylation mediated by phosphoenolpyruvate carboxykinase (PCK) in engineered Escherichia coli having high ATP / Enzyme Mi
Lee JY / 2014 / Artificial oxidative stress-tolerant Corynebacterium glutamicum / AMB Express 4 : 15 ~
Mauzerall D / 1956 / The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine / J. Biol. Chem 219 : 435 ~ 446
Min Ju Lee / 2012 / Porphyrin Derivatives from a Recombinant Escherichia coli Grown on Chemically Defined Medium / Journal of Microbiology and Biotechnology 22 (12) : 1653 ~
Lee HJ / 2013 / Estimation of phosphoenolpyruvate carboxylation mediated by phosphoenolpyruvate carboxykinase (PCK) in engineered Escherichia coli having high ATP / Enzyme Mi
Lee JY / 2014 / Artificial oxidative stress-tolerant Corynebacterium glutamicum / AMB Express 4 : 15 ~
Mauzerall D / 1956 / The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine / J. Biol. Chem 219 : 435 ~ 446
Min Ju Lee / 2012 / Porphyrin Derivatives from a Recombinant Escherichia coli Grown on Chemically Defined Medium / Journal of Microbiology and Biotechnology 22 (12) : 1653 ~
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