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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 271 - 275 (5page)
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The expression of Deinococcus radiodurans dr1558 and pprM genes was examined for enhanced lysine production in recombinant Corynebacterium glutamicum. These genes are known to confer high tolerance to pH and osmotic shock in Escherichia coli. D. radiodurans dr1558 and pprM genes were expressed in C.
glutamicum by using 6 synthetic promoters of different strengths, to evaluate the effect of expression efficiency on lysine production. Recombinant C. glutamicum expressing DR1558 under the L26 and I64 promoters showed higher lysine production than that expressing DR1558 under other promoters. Similarly, recombinant C. glutamicum expressing PprM under same promoters (L26 and I64) showed a higher increase in lysine production compared to that expressing PprM under other promoters. In the absence of CaCO3 in the medium, the expression of DR1558 or PprM also increased lysine concentration in C. glutamicum depending on the promoter used. Together, these results suggest that genes involved in radiation tolerance in D. radiodurans can be used to enhance production of amino acids and their derivatives.
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참고문헌 (22)
참고문헌 신청
Appukuttan D / 2016 / Engineering synthetic multistress tolerance in Escherichia coli by using a deinococcal response regulator, DR1558 / Appl. Environ. Microbiol 82 : 1154
Choi JW / 2015 / Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH rang
Fu RY / 2006 / Introducing glutathione biosynthetic capability into Lactococcus lactis subsp. cremoris NZ9000 improves the oxidative-stress resistance of the host / Metab. En
Ishino Y / 2015 / DNA repair in hyperthermophilic and hyperradioresistant microorganisms / Curr. Opin. Microbiol 25 : 103 ~ 112
Jeong Chan Joo / 2017 / Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly develo
Choi JW / 2015 / Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH rang
Fu RY / 2006 / Introducing glutathione biosynthetic capability into Lactococcus lactis subsp. cremoris NZ9000 improves the oxidative-stress resistance of the host / Metab. En
Ishino Y / 2015 / DNA repair in hyperthermophilic and hyperradioresistant microorganisms / Curr. Opin. Microbiol 25 : 103 ~ 112
Jeong Chan Joo / 2017 / Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly develo
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