지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Excessive drinking of alcohol is known to be one of the main causes of various neurological diseases, such as Alzheimer"s disease. Scopoletin is known to have anti-inflammatory and antioxidative properties, and to protect nerve cells. This study examined whether scopoletin inhibits the alcohol-induced apoptosis of primary hippocampal neurons, and how scopoletin regulates several factors associated with the caspase-mediated pathway. To achieve this, the cell viability and apoptosis rate of primary hippocampal neurons were measured by Cell Counting Kit-8 and flow cytometry, respectively. Apoptosis-related protein expressions (Bax, Bid, caspase-3, caspase-9, and Poly (ADP-ribose) polymerase (PARP)) were analyzed by Western blotting, and the ANOVA method was used to confirm the significance of the measured results. As a result, scopoletin inhibited the expressions of alcohol-induced apoptosis and apoptosis-related proteins in primary hippocampal neurons. These results suggest that down-regulation of Bid, Bax, and cleaved caspase-9 expression induced by scopoletin down-regulates the expression of cleaved caspase-3, inhibits the expression of cleaved PARP, and finally, inhibits mitochondrial apoptotic pathways. The study suggests that scopoletin is worth developing as a candidate for neuroprotective agent.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (38)
참고문헌 신청
Baloyannis SJ / 2006 / Mitochondrial alterations in alzheimer's disease / Journal of Alzheimer's Disease 9:119~126
Barker WW / 2002 / Relative frequencies of alzheimer disease, lewy body, vascular and frontotemporal dementia, and hippocampal sclerosis in the state of florida brain bank / Alzheimer Disease and Associated Disorders 16:203~212
Barnes DE / 2011 / The projected impact of risk factor reduction on alzheimer's disease prevalence / Lancet Neurology 10:819~828
Bonthius DJ / 1990 / Alcohol‐induced neuronal loss in developing rats : increased brain damage with binge exposure / Alcoholism : Clinical and Experimental Research 14:107~118
Calissano P / 2009 / Apoptosis and in vitro alzheimer's disease neuronal models / Communicative & Integrative Biology 2:163~169
Barker WW / 2002 / Relative frequencies of alzheimer disease, lewy body, vascular and frontotemporal dementia, and hippocampal sclerosis in the state of florida brain bank / Alzheimer Disease and Associated Disorders 16:203~212
Barnes DE / 2011 / The projected impact of risk factor reduction on alzheimer's disease prevalence / Lancet Neurology 10:819~828
Bonthius DJ / 1990 / Alcohol‐induced neuronal loss in developing rats : increased brain damage with binge exposure / Alcoholism : Clinical and Experimental Research 14:107~118
Calissano P / 2009 / Apoptosis and in vitro alzheimer's disease neuronal models / Communicative & Integrative Biology 2:163~169
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UCI(KEPA) : I410-ECN-0101-2020-510-000849708