Ginsenoside Rg3 and Korean Red Ginseng extract epigenetically regulate the tumor-related long noncoding RNAs RFX3-AS1 and STXBP5-AS1

Juyeon Ham; Dawoon Jeong; Sungbin Park; Hyeon Woo Kim; Heejoo Kim; Sun Jung Kim

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자료유형: 학술저널

저자정보: Juyeon Ham (Dongguk University-Seoul) Dawoon Jeong (Dongguk University-Seoul) Sungbin Park (Dongguk University-Seoul) Hyeon Woo Kim (Dongguk University-Seoul) Heejoo Kim (Dongguk University-Seoul) Sun Jung Kim (Dongguk University-Seoul)

저널정보: 고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.43 No.4

발행연도: 2019.10

수록면: 625 - 634 (10page)

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Background: Ginsenoside Rg3, a derivative of steroidal saponins abundant in ginseng, has a range of effects on cancer cells, including anti-cell proliferation and anti-inflammation activity. Here, we investigate two long noncoding RNAs (lncRNAs), STXBP5-AS1 and RFX3-AS1, which are hypomethylated and hypermethylated in the promoter region by Rg3 in MCF-7 cancer cells.
Methods: The lncRNAs epigenetically regulated by Rg3 were mined using methylation array analysis. The effect of the lncRNAs on the apoptosis and proliferation of MCF-7 cells was monitored in the presence of Rg3 or Korean Red Ginseng (KRG) extract after deregulating the lncRNAs. The expression of the lncRNAs and their target genes was examined using qPCR and Western blot analysis. The association between the expression of the target genes and the survival rate of breast cancer patients was analyzed using the Kaplan-Meier Plotter platform.
Results: STXBP5-AS1 and RFX3-AS1 exhibited anti- and pro-proliferation effects, respectively, in the cancer cells, and the effects of Rg3 and KRG extract on apoptosis and cell proliferation were weakened after deregulating the lncRNAs. Of the genes located close to STXBP5-AS1 and RFX3-AS1 on the chromosome, STXBP5, GRM1, RFX3, and SLC1A1 were regulated by the lncRNAs on the RNA and protein level. Breast cancer patients that exhibited a higher expression of the target genes of the lncRNAs had a higher metastasis-free survival rate.
Conclusion: The current study is the first to identify lncRNAs that are regulated by the presence of Rg3 and KRG extract and that subsequently contribute to inhibiting the proliferation of cancer cells.

#CpG methylation #ginsenoside Rg3 #long noncoding RNA #Korean Red Ginseng

참고문헌 (29)

참고문헌 신청

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Wang JH / 2014 / 20(s)-ginsenoside Rg3 promotes apoptosis in human ovarian cancer HO-8910 cells through PI3K/Akt and XIAP pathways / Tumour Biol 35:11985~11994

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