Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

Gulcin Ercan; Rumeysa İlbar Tartar; Ali Solmaz; Osman Bilgin Gulcicek; Onur Olgac Karagulle; Serhat Meric; Huseyin Cayoren; Ramazan Kusaslan; Ahu Kemik; Damla Gokceoglu Kayali; Sule Cetinel; Atilla Celik

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저자정보: Gulcin Ercan (University of Health Sciences Bagcilar Training and Research Hospital) Rumeysa İlbar Tartar (University of Health Sciences) Ali Solmaz (University of Health Sciences Bagcilar Training and Research Hospital) Osman Bilgin Gulcicek (University of Health Sciences Bagcilar Training and Research Hospital) Onur Olgac Karagulle (Buyukcekmece Mimar Sinan State Hospital) Serhat Meric (University of Health Sciences Bagcilar Training and Research Hospital) Huseyin Cayoren (Igdir State Hospital) Ramazan Kusaslan (Elitium Surgery Center) Ahu Kemik (Istanbul University) Damla Gokceoglu Kayali (Marmara University) Sule Cetinel (Marmara University) Atilla Celik (University of Health Sciences Bagcilar Training and Research Hospital)

저널정보: 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.97 No.6

발행연도: 2019.12

수록면: 271 - 281 (11page)

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Purpose: To determine the potential protective and therapeutic effects and action mechanism of ruscogenin on cerulein-induced acute pancreatitis (AP) model in rats.
Methods: Overall, 32 rats were attenuated to the sham (2-mL/kg/day isotonic solution for 4 weeks), control (20-μg/kg cerulein-induced AP for 12 hours), prophylaxis groups (cerulein-induced AP following 3-mL/kg/day ruscogenin for 4 weeks) and treatment (3-mL/kg/day ruscogenin following cerulein-induced AP for 12 hours). Blood samples were collected for biochemical analysis of nitric oxide synthase 1 (NOS1/neuronal NOS), malondialdehyde (MDA) and intercellular adhesion molecule 1 (ICAM-1). After sacrification, pancreas tissues were collected and prepared for light microscopic (hematoxylin and eosin), immunohistochemical (nuclear factor kappa B) and biochemical analysis (tumor necrosis factor-alpha [TNF-α], interleukin-6 and 1β [IL-6 and IL-1β], CRP, high-sensitivity CRP [hs-CRP] amylase, lipase, and ICAM-1). Ultrastructural analysis was performed by transmission electron microscopy.
Results: The protective and therapeutic actions of ruscogenin were accomplished by improvements in histopathology, by decreasing blood cytokine levels of CRP, hs-CRP levels, TNF-α, IL-6, IL-1β, ICAM-1, by reducing the pancreatic enzymes amylase and lipase in blood, and by suppressing the expression of nuclear factor kappa B, ICAM-1, and NOS-1, but not MDA in pancreatic tissues. Ruscogenin also improved cerulein-induced ultrastructural degenerations in endocrine and exocrine cells, especially in treatment group.
Conclusion: The present findings have demonstrated the beneficial protective and therapeutical effects of ruscogenin, nominating it as a highly promising supplementary agent to be considered in the treatment of AP, and even as a protective agent against the damages induced by disease.

#Ceruletide #NF-kappa B #Pancreas #Pancreatitis #Ruscogenin

참고문헌 (30)

참고문헌 신청

김혜영 / 2008 / Cerulein Pancreatitis: Oxidative Stress, Inflammation, and Apoptosis / Gut and Liver 2(2):74~80

Fisic E / 2013 / role of IL-6, 8, and 10, sTNFr, CRP, and pancreatic elastase in the prediction of systemic complications in patients with acute pancreatitis / Gastroenterol Res Pract 2013:282645

McKay CJ / 1996 / Increased monocyte cytokine production in association with systemic complications in acute pancreatitis / Br J Surg 83:919~923

Al-Bahrani AZ / 2005 / Clinical laboratory assessment of acute pancreatitis / Clin Chim Acta 362:26~48

Schoenberg MH / 1990 / Oxygen free radicals in acute pancreatitis of the rat / Gut 31:1138~1143

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