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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 250 - 258 (9page)
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초록· 키워드
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Peniphora sp. JS17, isolated from forest old tree, produced extracellular enzymes that decolorized human hair melanin. The JS17 strain had laccase and manganese peroxidase activity while it did not has lignin peroxidase activity. Batch culture indicated that the melanin decolorization activity of JS17 strain originated from laccase. The culture conditions to maximize the production of melanin bleaching enzymes from Peniophora sp. JS17 mycelia were investigated. Among the tested media for the laccase production, minimal medium (2% glucose, 0.2% malt extract, 0.1% KH2PO4, 0.4% MgSO4·7H2O) showed the highest activity of laccase. Then, to optimize the culture condition for the laccase activity, the influence of various carbon and nitrogen sources was investigated in minimal medium. Among various carbon and nitrogen sources, 2% xylose and 0.4% tryptone showed the highest production of laccase, respectively. The enzyme was purified using (NH4)2SO4 precipitation and Hitrap Q sepharose column, and the purified enzyme showed two isoenzymatic bands with molecular masses of about 70 kDa by SDS-PAGE. The melanin decolorization activity was 77% and 55% within 48 h in the presence of 1-hydroxybenzotriazole (HBT) and syringaldehyde, respectively, whereas only about 9% melanin decolorized in case of no mediator.
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참고문헌 (32)
참고문헌 신청
Lin WP / 2005 / Effect of melanin produced by a recombinant Escherichia coli on antibacterial activity of antibiotics / J. Microbiol. Immunol. Infect 38:320~326
Butler MJ / 1998 / Destruction of fungal melanins by ligninases of Phanerochaete chrysosporium and other white rot fungi / Int. J. Plant Sci 159:989~995
Butler MJ / 1998 / Fungal melanins: a review / Can. J. Microbiol 44:1115~1136
Riley PA / 1997 / Melanin / Int. J. Biochem. Cell Biol 29:1235~1239
Henson JM / 2001 / Biopolymers, vol.1 / Wiley-VCH Verlag GmbH :229~246
Butler MJ / 1998 / Destruction of fungal melanins by ligninases of Phanerochaete chrysosporium and other white rot fungi / Int. J. Plant Sci 159:989~995
Butler MJ / 1998 / Fungal melanins: a review / Can. J. Microbiol 44:1115~1136
Riley PA / 1997 / Melanin / Int. J. Biochem. Cell Biol 29:1235~1239
Henson JM / 2001 / Biopolymers, vol.1 / Wiley-VCH Verlag GmbH :229~246
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