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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제5호
- 발행연도
- 2015.1
- 수록면
- 718 - 722 (5page)
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초록· 키워드
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Techniques for immobilizing effective enzymes on nanoparticles for stabilization of the activity of free enzymes have been developing as a pharmaceutical field. In this study, we examined the effect of three different pH conditions of phosphate buffer, as a dissolving solvent for lysosomal enzymes, on the direct immobilization of lysosomal enzymes extracted from Hen’s egg white and Saccharomyces cerevisiae. Titanium(IV) oxide (TiO2) nanoparticles, which are extensively used in many research fields, were used in this study. The lysosomal enzymes immobilized on TiO2 under each pH condition were evaluated to maintain the specific activity of lysosomal enzymes, so that we can determine the degree of melanin treatment in lysosomal enzymes immobilized on TiO2. We found that the immobilization efficiency and melanin treatment activity in both lysosomal enzymes extracted from Hen’s egg white and S. cerevisiae were the highest in an acidic condition of phosphate buffer (pH 4). However, the immobilization efficiency and melanin treatment activity were inversely proportional to the increase in pH under alkaline conditions. In addition, enhanced immobilization efficiency was shown in TiO2 pretreated with a divalent, positively charged ion, Ca2+, and the melanin treatment activity of immobilized lysosomal enzymes on TiO2 pretreated with Ca2+ was also increased. Therefore, this result suggests that the immobilization efficiency and melanin treatment activity of lysosomal enzymes can be enhanced according to the pH conditions of the dissolving solvent.
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참고문헌 (20)
참고문헌 신청
Benkstein KD / 2006 / Mesoporous nanoparticle TiO2 thin films for conductometric sensing on microhotplate platforms / Sens. Actuator B Chem 113 : 445 ~ 453
Choi H / 2007 / Photocatalytic TiO2 films and membranes for the development of efficient wastewater treatment and reuse systems / Desalination 202 : 199 ~ 206
Cirman T / 2004 / Selective disruption of lysosomes in Hela cells triggers apoptosis mediated by cleavage of Bid by multiple papain-like lysosomal cathepsins / J. Biol. Chem
Deiss LP / 1996 / Cathepsin D protease mediates programmed cell death induced by interferon-gamma, Fas/APO-1 and TNF-alpha / EMBO J 15 : 3861 ~ 3870
Dell’angelica EC / 2000 / Lysosome-related organellse / FASEB J 14 : 1265 ~ 1278
Choi H / 2007 / Photocatalytic TiO2 films and membranes for the development of efficient wastewater treatment and reuse systems / Desalination 202 : 199 ~ 206
Cirman T / 2004 / Selective disruption of lysosomes in Hela cells triggers apoptosis mediated by cleavage of Bid by multiple papain-like lysosomal cathepsins / J. Biol. Chem
Deiss LP / 1996 / Cathepsin D protease mediates programmed cell death induced by interferon-gamma, Fas/APO-1 and TNF-alpha / EMBO J 15 : 3861 ~ 3870
Dell’angelica EC / 2000 / Lysosome-related organellse / FASEB J 14 : 1265 ~ 1278
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