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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 280 - 287 (8page)
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초록· 키워드
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In this study, transcriptome analysis of twelve laccase genes in Pleurotus ostreatus revealed that their expression was differentially regulated at different developmental stages. Lacc5 and Lacc12 were specifically expressed in fruiting bodies and primordia, respectively, whereas Lacc6 was expressed at all developmental stages. Lacc1 and Lacc3 were specific to the mycelial stage in solid medium. In order to investigate their biochemical characteristics, these laccases were heterologously expressed in Pichia pastoris using the pPICHOLI-2 expression vector. Expression of the laccases was facilitated by intermittent addition of methanol as an inducer and sole carbon source, in order to reduce the toxic effects associated with high methanol concentration. The highest expression was observed when the recombinant yeast cells were grown for 5 days at $15^{\circ}C$ with intermittent addition of 1% methanol at a 12-hr interval. Investigation of enzyme kinetics using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) as a substrate revealed that the primordium-specific laccase Lacc12 was 5.4-fold less active than Lacc6 at low substrate concentration with respect to ABTS oxidation activity. The optimal pH and temperature of Lacc12 were 0.5 pH units and $5^{\circ}C$higher than those of Lacc6. Lacc12 showed maximal activity at pH 3.5 and $50^{\circ}C$, which may reflect the physiological conditions at the primordiation stage.
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참고문헌 (36)
참고문헌 신청
Wang Y / 2013 / Plant cell wall lignification and monolignol metabolism / Front Plant Sci 4:220
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Majcherczyk A / 1998 / Oxidation of polycyclic aromatic hydrocarbons (PAH) by laccase of Trametes versicolor / Enzyme Microb Technol 22:335~341
Park JW / 2015 / Strain-dependent response to Cu2+ in the expression of laccase in Pycnoporus coccineus / Arch Microbiol 197:589~596
Hyeon-Woo Kang / 2014 / Decolorization of Triphenylmethane Dyes by Wild Mushrooms / Biotechnology and Bioprocess Engineering 19(3):519~525
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Majcherczyk A / 1998 / Oxidation of polycyclic aromatic hydrocarbons (PAH) by laccase of Trametes versicolor / Enzyme Microb Technol 22:335~341
Park JW / 2015 / Strain-dependent response to Cu2+ in the expression of laccase in Pycnoporus coccineus / Arch Microbiol 197:589~596
Hyeon-Woo Kang / 2014 / Decolorization of Triphenylmethane Dyes by Wild Mushrooms / Biotechnology and Bioprocess Engineering 19(3):519~525
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