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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Onion peel extract and its constituent, quercetin inhibits human Slo3 in a pH and calcium dependent manner
Onion peel extract and its constituent, quercetin inhibits human Slo3 in a pH and calcium dependent manner
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제23권 제5호 KCI Accredited Journals
- 발행연도
- 2019.1
- 수록면
- 381 - 392 (12page)
이용수
Onion peel extract and its constituent, quercetin inhibits human Slo3 in a pH and calcium dependent manner
초록· 키워드
오류제보하기
Sperm function and male fertility are closely related to pH dependent K+ current (KSper) in human sperm, which is most likely composed of Slo3 and its auxiliary subunit leucine-rich repeat-containing protein 52 (LRRC52). Onion peel extract (OPE) and its major active ingredient quercetin are widely used as fertility enhancers; however, the effect of OPE and quercetin on Slo3 has not been elucidated. The purpose of this study is to investigate the effect of quercetin on human Slo3 channels. Human Slo3 and LRRC52 were co-transfected into HEK293 cells and pharmacological properties were studied with the whole cell patch clamp technique. We successfully expressed and measured pH sensitive and calcium insensitive Slo3 currents in HEK293 cells. We found that OPE and its key ingredient quercetin inhibit Slo3 currents. Inhibition by quercetin is dose dependent and this degree of inhibition decreases with elevating internal alkalization and internal free calcium concentrations. Functional moieties in the quercetin polyphenolic ring govern the degree of inhibition of Slo3 by quercetin, and the composition of such functional moieties are sensitive to the pH of the medium. These results suggest that quercetin inhibits Slo3 in a pH and calcium dependent manner. Therefore, we surmise that quercetin induced depolarization in spermatozoa may enhance the voltage gated proton channel (Hv1), and activate non-selective cation channels of sperm (CatSper) dependent calcium influx to trigger sperm capacitation and acrosome reaction.
목차
등록된 정보가 없습니다.
참고문헌 (42)
참고문헌 신청
Lishko PV / 2010 / Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel / Cell 140:327~337
Flesch FM / 2000 / Dynamics of the mammalian sperm plasma membrane in the process of fertilization / Biochim Biophys Acta 1469:197~235
Baldi E / 1991 / Intracellular calcium accumulation and responsiveness to progesterone in capacitating human spermatozoa / J Androl 12:323~330
Kirichok Y / 2011 / Rediscovering sperm ion channels with the patch-clamp technique / Mol Hum Reprod 17:478~499
Lishko PV / 2012 / The control of male fertility by spermatozoan ion channels / Annu Rev Physiol 74:453~475
Flesch FM / 2000 / Dynamics of the mammalian sperm plasma membrane in the process of fertilization / Biochim Biophys Acta 1469:197~235
Baldi E / 1991 / Intracellular calcium accumulation and responsiveness to progesterone in capacitating human spermatozoa / J Androl 12:323~330
Kirichok Y / 2011 / Rediscovering sperm ion channels with the patch-clamp technique / Mol Hum Reprod 17:478~499
Lishko PV / 2012 / The control of male fertility by spermatozoan ion channels / Annu Rev Physiol 74:453~475
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