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- 학술저널
- 저자정보
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- 2022.3
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이 논문의 연구 히스토리 (3)
2022
Quercetin prevents glutamate toxicity-induced neuronal cell damage by regulating parvalbumin expression
Ju-Bin Kang
,
Dong-Ju Pa
,
k
외 3명
한국실험동물학회 학술발표대회 논문집
2022.07
학술대회자료
[Molecular and Cellular Biology] Quercetin ameliorates glutamate toxicity-induced neuronal cell death by controlling calcium-binding protein parvalbumin
Ju-Bin Kang
,
Dong-Ju Pa
,
k
외 3명
Journal of Veterinary Science
2022.03
학술저널
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Background: Glutamate is the main excitatory neurotransmitter. Excessive glutamate causes excitatory toxicity and increases intracellular calcium, leading to neuronal death. Parvalbumin is a calcium-binding protein that regulates calcium homeostasis. Quercetin is a polyphenol found in plant and has neuroprotective effects against neurodegenerative diseases.
Objectives: We investigated whether quercetin regulates apoptosis by modulating parvalbumin expression in glutamate induced neuronal damage.
Methods: Glutamate was treated in hippocampal-derived cell line, and quercetin or vehicle was treated 1 h before glutamate exposure. Cells were collected for experimental procedure 24 h after glutamate treatment and intracellular calcium concentration and parvalbumin expression were examined. Parvalbumin small interfering RNA (siRNA) transfection was performed to detect the relation between parvalbumin and apoptosis.
Results: Glutamate reduced cell viability and increased intracellular calcium concentration, while quercetin preserved calcium concentration and neuronal damage. Moreover, glutamate reduced parvalbumin expression and quercetin alleviated this reduction. Glutamate increased caspase-3 expression, and quercetin attenuated this increase in both parvalbumin siRNA transfected and non-transfected cells. The alleviative effect of quercetin was statistically significant in non-transfected cells. Moreover, glutamate decreased bcl-2 and increased bax expressions, while quercetin alleviated these changes. The alleviative effect of quercetin in bcl-2 family protein expression was more remarkable in non-transfected cells.
Conclusions: These results demonstrate that parvalbumin contributes to the maintainace of intracellular calcium concentration and the prevention of apoptosis, and quercetin modulates parvalbumin expression in glutamate-exposed cells. Thus, these findings suggest that quercetin performs neuroprotective function against glutamate toxicity by regulating parvalbumin expression.
Objectives: We investigated whether quercetin regulates apoptosis by modulating parvalbumin expression in glutamate induced neuronal damage.
Methods: Glutamate was treated in hippocampal-derived cell line, and quercetin or vehicle was treated 1 h before glutamate exposure. Cells were collected for experimental procedure 24 h after glutamate treatment and intracellular calcium concentration and parvalbumin expression were examined. Parvalbumin small interfering RNA (siRNA) transfection was performed to detect the relation between parvalbumin and apoptosis.
Results: Glutamate reduced cell viability and increased intracellular calcium concentration, while quercetin preserved calcium concentration and neuronal damage. Moreover, glutamate reduced parvalbumin expression and quercetin alleviated this reduction. Glutamate increased caspase-3 expression, and quercetin attenuated this increase in both parvalbumin siRNA transfected and non-transfected cells. The alleviative effect of quercetin was statistically significant in non-transfected cells. Moreover, glutamate decreased bcl-2 and increased bax expressions, while quercetin alleviated these changes. The alleviative effect of quercetin in bcl-2 family protein expression was more remarkable in non-transfected cells.
Conclusions: These results demonstrate that parvalbumin contributes to the maintainace of intracellular calcium concentration and the prevention of apoptosis, and quercetin modulates parvalbumin expression in glutamate-exposed cells. Thus, these findings suggest that quercetin performs neuroprotective function against glutamate toxicity by regulating parvalbumin expression.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
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Quercetin prevents glutamate toxicity-induced neuronal cell damage by regulating parvalbumin expression
한국실험동물학회 학술발표대회 논문집
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Quercetin reduces the injury-induced reduction of γ-enolase expression in a middle cerebral artery occlusion animal model
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Preventive and Therapeutic Effects of Quercetin on Experimental Radiation Induced Lung Injury in Mice
Asian Pacific journal of cancer prevention : APJCP
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Quercetin induces cell death by caspase-dependent and p38 MAPK pathway in EGFR mutant lung cancer cells
고신대학교 의과대학 학술지
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Autophagy Inhibition Promotes Quercetin Induced Apoptosis in MG-63 Human Osteosarcoma cells
International Journal of Oral Biology
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Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity
The Korean Journal of Physiology & Pharmacology
2017 .03
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Quercetin Directly Interacts with Vitamin D Receptor (VDR): Structural Implication of VDR Activation by Quercetin
Biomolecules & Therapeutics(구 응용약물학회지)
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Effect of parvalbumin-positive neurons in the main olfactory bulb following lithium pilocarpine-induced status epilepticus in rat
대한체질인류학회 학술대회 연제 초록
2016 .05
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급성 일산화탄소 중독 후 해마에서 Parvalbumin 양성 중간뉴론의 변화에 대한 N-acetylcystein의 효과
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HepG2 cell에서 quercetin의 HO-1 발현을 통한 mitochondria의 생합성 유도 효과에 관한 연구
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2015 .01
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Effect of proton beam irradiation on cytotoxicity and anti-inflammatory activity of quercetin
한국실험동물학회 학술발표대회 논문집
2016 .08
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Onion peel extract and its constituent, quercetin inhibits human Slo3 in a pH and calcium dependent manner
The Korean Journal of Physiology & Pharmacology
2019 .01
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Quercetin Upregulates Uncoupling Protein 1 in White/Brown Adipose Tissues through Sympathetic Stimulation
Journal of Obesity & Metabolic Syndrome
2018 .01
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