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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제9호
- 발행연도
- 2017.1
- 수록면
- 1,586 - 1,592 (7page)
이용수
초록· 키워드
오류제보하기
Some promoters were isolated and characterized from the genome of Leuconostoc mesenteroides SY2, an isolate from kimchi, a Korean traditional fermented vegetable. Chromosomal DNA of L. mesenteroides SY2 was digested with Sau3AI and ligated with BamHI-cut pBV5030, a promoter screening vector containing a promoterless cat-86. Among E. coli transformants (TFs) resistant against Cm (chloramphenicol), 17 were able to grow in the presence of 1,000 μg/ml Cm and their inserts were sequenced. Transcription start sites were examined for three putative promoters (P04C, P25C, and P33C) by primer extension. Four putative promoters were inserted upstream of a promoterless α-amylase reporter gene in pJY15α. α-Amylase activities of E. coli TFs containing pJY15α (control, no promoter), pJY03α (pJY15α with P03C), pJY04α (with P04C), pJY25α (with P25C), and pJY33α (with P33C) were 66.9, 78.7, 122.1, 70.8, and 99.3 U, respectively. Cells harboring pJY04α showed 1.8 times higher activity than the control. Some promoters characterized in this study might be useful for construction of foodgrade expression vectors for Leuconostoc sp. and related lactic acid bacteria.
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참고문헌 (22)
참고문헌 신청
Caplice E / 1999 / Food fermentations: role of microorganisms in food production and preservation / Int. J. Food Microbiol 50:131~149
Settanni L / 2008 / Application of bacteriocins in vegetable food biopreservation / Int. J. Food Microbiol 121:123~138
Morello E / 2008 / Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion / J. Mol. Microbiol. Biotechnol 14:48~58
Thakur K / 2016 / Lactic acid bacteria as a cell factory for riboflavin production / Microb. Biotechnol 9:441~451
Kleerebezem M / 2003 / Metabolic pathway engineering in lactic acid bacteria / Curr. Opin. Biotechnol 14:232~237
Settanni L / 2008 / Application of bacteriocins in vegetable food biopreservation / Int. J. Food Microbiol 121:123~138
Morello E / 2008 / Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion / J. Mol. Microbiol. Biotechnol 14:48~58
Thakur K / 2016 / Lactic acid bacteria as a cell factory for riboflavin production / Microb. Biotechnol 9:441~451
Kleerebezem M / 2003 / Metabolic pathway engineering in lactic acid bacteria / Curr. Opin. Biotechnol 14:232~237
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