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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제11호
- 발행연도
- 2017.1
- 수록면
- 1,971 - 1,982 (12page)
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초록· 키워드
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Piliated Lactobacillus rhamnosus (pLR) strains possess higher adherent capacity than non-piliated strains. The objective of this study was to isolate and characterize probiotic pLR strains in human fecal samples. To this end, mouse polyclonal antiserum (anti-SpaA) against the recombinant pilus protein (SpaA) of L. rhamnosus strain GG (LGG) was prepared and tested for its reactivity and specificity. With the anti-SpaA, a method combining the de Man, Rogosa, and Sharpe (MRS) agar plating separation and colony immunoblotting (CIB) was developed to isolate pLR from 124 human fecal samples. The genetic and phenotypic characteristics of the resultant pLR isolates were compared by randomly amplified polymorphic DNA (RAPD) fingerprinting, and examination of adhesion to Caco-2 cells, hydrophobicity, autoaggregation, and in vitro gastrointestinal tolerance. Anti-SpaA specifically reacted with three pLR strains of 25 test strains, as assessed by western blotting, immunofluorescence flow cytometry, and immunoelectron microscopy (IEM) assays. The optimized MRS agar separation plus anti- SpaA-based CIB procedure could quantitatively detect 2.5 × 103 CFU/ml of pLR colonies spiked in 106 CFU/ml of background bacteria. Eight pLR strains were identified in 124 human fecal samples, and were confirmed by 16S RNA gene sequencing and IEM identification.
RAPD fingerprinting of the pLR strains revealed seven different patterns, of which only two isolates from infants showed the same RAPD profiles with LGG. Strain PLR06 was obtained with high adhesion and autoaggregation activities, hydrophobicity, and gastrointestinal tolerance. Anti-SpaA-based CIB is a rapid and inexpensive method for the preliminary screening of novel adherent L. rhamnosus strains for commercial purposes.
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참고문헌 (22)
참고문헌 신청
[학술지(정기간행물)
Alander M / 1999 / Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption / Appl. Environ. Microbiol 65
Belyi YF / 1995 / A simple colony-blot method for identification of Listeria in food samples / Med. Microbiol. Immunol 184 : 105 ~ 108
Bullens DM / 2015 / Piliation of Lactobacillus rhamnosus GG promotes adhesion, phagocytosis, and cytokine modulation in macrophages / Appl. Environ. Microbiol 81 : 2050 ~ 20
Doron S / 2005 / Lactobacillus GG:bacteriology and clinical applications / Gastroenterol. Clin. North Am 34 : 483 ~ 498
Alander M / 1999 / Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption / Appl. Environ. Microbiol 65
Belyi YF / 1995 / A simple colony-blot method for identification of Listeria in food samples / Med. Microbiol. Immunol 184 : 105 ~ 108
Bullens DM / 2015 / Piliation of Lactobacillus rhamnosus GG promotes adhesion, phagocytosis, and cytokine modulation in macrophages / Appl. Environ. Microbiol 81 : 2050 ~ 20
Doron S / 2005 / Lactobacillus GG:bacteriology and clinical applications / Gastroenterol. Clin. North Am 34 : 483 ~ 498
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