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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제9호
- 발행연도
- 2009.1
- 수록면
- 987 - 996 (10page)
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초록· 키워드
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Aspergillus niger GH1 previously isolated and identified
by our group as a wild tannase producer was grown under
solid-state (SSC) and submerged culture (SmC) conditions
to select the enzyme production system. For tannase
purification, extracellular tannase was produced under
SSC using polyurethane foam as the inert support. Tannase
was purified to apparent homogeneity by ultrafiltration,
anion-exchange chromatography, and gel filtration that led
to a purified enzyme with a specific activity of 238.14 IU/mg
protein with a final yield of 0.3% and a purification fold of
46. Three bands were found on the SDS-PAG with molecular
masses of 50, 75, and 100 kDa. PI of 3.5 and 7.1% Nglycosylation
were noted. Temperature and pH optima were
60oC and 6.0 [methyl 3,4,5-trihydroxybenzoate (MTB) as
substrate], respectively. Tannase was found with a KM value
of 0.41×10-4 M and the value of Vmax was 11.03 μmoL/min
at 60oC for MTB. Effects of several metal salts, solvents,
surfactants, and typical enzyme inhibitors on tannase
activity were evaluated to establish the novelty of the
enzyme. Finally, the tannase from A. niger GH1 was
significantly inhibited by PMSF (phenylmethylsulfonyl
fluoride), and therefore, it is possible to consider the presence
of a serine or cysteine residue in the catalytic site.
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참고문헌 (24)
참고문헌 신청
Adachi, O. / 1971 / Physicochemical properties of the tannase from Aspergillus flavus / Agric. Biol. Chem 32 : 1079 ~ 1085
Aguilar, C. N. / 1999 / A comparision of methods to determine tannin acyl hydrolase activity / Braz. Arch. of Bio. Tech 42 : 355 ~ 361
Aguilar, C. N. / 2001 / Review: Sources, properties, applications and potential uses of tannin acyl hydrolase / Food Sci. Technol. Int 7 : 373 ~ 382
Aguilar, C. N. / 2007 / Microbial tannases: Advances and perspectives / Appl. Microbiol. Biotechnol 76 : 47 ~ 59
Aguilar, C. N. / 2008 / Production of antioxidant-nutraceuticals by solid state cultures of pomegranate residues (Punica granatum) and creosote bush (Larrea tridentata) / Foo
Aguilar, C. N. / 1999 / A comparision of methods to determine tannin acyl hydrolase activity / Braz. Arch. of Bio. Tech 42 : 355 ~ 361
Aguilar, C. N. / 2001 / Review: Sources, properties, applications and potential uses of tannin acyl hydrolase / Food Sci. Technol. Int 7 : 373 ~ 382
Aguilar, C. N. / 2007 / Microbial tannases: Advances and perspectives / Appl. Microbiol. Biotechnol 76 : 47 ~ 59
Aguilar, C. N. / 2008 / Production of antioxidant-nutraceuticals by solid state cultures of pomegranate residues (Punica granatum) and creosote bush (Larrea tridentata) / Foo
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